Anti-REST / NRSF 抗体 (ab21635)
Key features and details
- Rabbit polyclonal to REST / NRSF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-REST / NRSF antibody
REST / NRSF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to REST / NRSF -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Mouse REST/ NRSF aa 1050 to the C-terminus (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab17042) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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KO cell lines
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KO cell lysates
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21635の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa). |
ターゲット情報
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機能
Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. -
組織特異性
Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary. -
配列類似性
Contains 9 C2H2-type zinc fingers. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5978 Human
- Entrez Gene: 19712 Mouse
- Entrez Gene: 83618 Rat
- Omim: 600571 Human
- SwissProt: Q13127 Human
- SwissProt: Q155C6 Mouse
- SwissProt: Q8VIG1 Mouse
- SwissProt: O54963 Rat
see all -
別名
- Neural Restrictive Silencer Factor antibody
- Neural-restrictive silencer factor antibody
- Neuron Restrictive Silencer Factor antibody
see all
画像
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Western blot - Anti-REST / NRSF antibody (ab21635)All lanes : Anti-REST / NRSF antibody (ab21635) at 1 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : Wild-type HeLa Vehicle Control DFO (0mM, 16h) Nuclear cell lysate
Lane 3 : Wild-type HeLa Treated DFO (0.5mM, 16h) Nuclear cell lysate
Lane 4 : REST knockout HeLa Vehicle Control DFO (0mM, 16h) Nuclear cell lysate
Lane 5 : REST knockout HeLa Treated DFO (0.5mM, 16h) Nuclear cell lysate
Lane 6 : ES-D3 Nuclear Fraction cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 121 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-REST / NRSF antibody staining at 1 ug/ml, shown in green; Mouse anti SIRT1 nuclear loading control staining at 1/20000 dilution, shown in red. In Western blot, ab21635 was shown to bind specifically to REST / NRSF. A band was observed at 180 kDa in wild-type HeLa cell lysates with no signal observed at this size in resT CRISPR-Cas9 edited cell line ab265017 (CRISPR-Cas9 edited cell lysate ab257636). The band observed in the CRISPR-Cas9 edited lysate lane below 180 kDa is likely to represent a truncated form of REST / NRSF. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and resT CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (29)
ab21635 は 29 報の論文で使用されています。
- Bebber CM et al. Ferroptosis response segregates small cell lung cancer (SCLC) neuroendocrine subtypes. Nat Commun 12:2048 (2021). PubMed: 33824345
- Singh NP et al. Genome-Wide Binding Analyses of HOXB1 Revealed a Novel DNA Binding Motif Associated with Gene Repression. J Dev Biol 9:N/A (2021). PubMed: 33546292
- Rossi C et al. Cell Stress Induces Mislocalization of Transcription Factors with Mitochondrial Enrichment. Int J Mol Sci 22:N/A (2021). PubMed: 34445555
- Bray JK et al. Loss of RE-1 silencing transcription factor accelerates exocrine damage from pancreatic injury. Cell Death Dis 11:138 (2020). PubMed: 32080178
- Xie Y & Cheng Y LINC01198 facilitates gliomagenesis through activating PI3K/AKT pathway. RNA Biol 17:1040-1052 (2020). PubMed: 32378450