Anti-Reptin/TIP49B/RUVB2 抗体 [EPR4146] (ab108962)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4146] to Reptin/TIP49B/RUVB2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Reptin/TIP49B/RUVB2 antibody [EPR4146]
Reptin/TIP49B/RUVB2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4146] to Reptin/TIP49B/RUVB2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HepG2, Daudi, HeLa, and SKBR-3 cell lysates
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR4146 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108962の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa). |
ターゲット情報
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機能
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. -
組織特異性
Ubiquitously expressed. Highly expressed in testis and thymus. -
配列類似性
Belongs to the ruvB family. -
ドメイン
The C-terminal domain is required for association with ATF2. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes. - Information by UniProt
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参照データベース
- Entrez Gene: 10856 Human
- Entrez Gene: 20174 Mouse
- Omim: 604788 Human
- SwissProt: Q9Y230 Human
- SwissProt: Q9WTM5 Mouse
- Unigene: 515846 Human
- Unigene: 34410 Mouse
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別名
- 48 kDa TATA box-binding protein-interacting protein antibody
- 48 kDa TBP-interacting protein antibody
- 48-kDa TATA box-binding protein-interacting protein antibody
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画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab108962 は 1 報の論文で使用されています。
- Satkunanathan S et al. Establishment of a novel cell line for the enhanced production of recombinant adeno-associated virus vectors for gene therapy. Hum Gene Ther 25:929-41 (2014). WB . PubMed: 25072415