Application
Western blot
Sample
Arabidopsis thaliana Tissue lysate - whole (10 day old seedlings, grown on MS medium)
Gel Running Conditions
Reduced Denaturing (8% gel)
Loading amount
100 µg
Specification
10 day old seedlings, grown on MS medium
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Goat IgG (whole molecule)–Peroxidase antibody
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: No kDa Non-specific: 70 kDa
Positive control
I do not have positive control for the antibody but for chemi-luminescence reaction development I used chemi-luminescence compatible protein ladder which showed signal.
Additional data
Additional Notes
Protein extraction buffer: RIPA buffer with complete mini protease inhibitor (Roche)
Membrane: PVDF
Washing buffer: PBST
Concentration of protein tested : 10-100 ug
Dilution of primary antibody tested: 1/1000, 1/2500, 1/5000
Abcam response
Arabidopsis thaliana was tested as part of our AbTrial Program. Please contact our Scientific Support group for more information on this testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 22 2015