Anti-Ref2P 抗体 (ab178440)
Key features and details
- Rabbit polyclonal to Ref2P
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
製品の概要
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製品名
Anti-Ref2P antibody -
製品の詳細
Rabbit polyclonal to Ref2P -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Drosophila melanogaster -
免疫原
Synthetic peptide corresponding to Drosophila melanogaster Ref2P aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: P14199 -
ポジティブ・コントロール
- This antibody gave a positive signal in Atg8a mutant Fruit Flies (D.melanogaster).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab178440の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 65 kDa.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 65 kDa. |
ターゲット情報
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機能
Implicated in sigma rhabdovirus multiplication and necessary for male fertility. -
配列類似性
Contains 1 OPR domain.
Contains 1 UBA domain.
Contains 1 ZZ-type zinc finger. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 35246 Drosophila melanogaster
- SwissProt: P14199 Drosophila melanogaster
- Unigene: 12210 Drosophila melanogaster
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別名
- Protein ref(2)P antibody
- ref(2)P antibody
- REF2P_DROME antibody
- Refractory to sigma P antibody
画像
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All lanes : Anti-Ref2P antibody (ab178440) at 1/500 dilution (Blocking 60 minutes in TBS+0.1% Tween-20 + 5% non-fat milk)
Lane 1 : Wt adult drosophila lysate
Lane 2 : Atg8a mutant adult drosophila lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-rabbit HRP at 1/10000 dilution
Predicted band size: 65 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsGel 8% acrylamide, transferred onto PVDF membrane for 60 minutes at 100V in 10% ethanol buffer. Anti-Ref2P antibody 1:500 incubated overnight at 4ºC, with gentle agitation in TBS + 0.1% Tween-20.
The genotype of the Atg8a mutant flies used is: Atg8aKG07569
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All lanes : Anti-Ref2P antibody (ab178440) at 1/100 dilution (Milk blocked)
Lanes 1-3 : Atg8a mutant fruit flies (D.melanogaster)
Lanes 4-6 : Fruit flies (D.melanogaster) expressing ubiquitously Ref(2)p RNAi
Predicted band size: 65 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted?The bands observed at the bottom of the gel represent Tubulin and were used as a loading control within this experiment.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (39)
ab178440 は 39 報の論文で使用されています。
- Wang R et al. ESCRT dysfunction compromises endoplasmic reticulum maturation and autophagosome biogenesis in Drosophila. Curr Biol 32:1262-1274.e4 (2022). PubMed: 35134326
- Nassari S et al. Rab21 in enterocytes participates in intestinal epithelium maintenance. Mol Biol Cell 33:ar32 (2022). PubMed: 35171715
- Rahman A et al. GMAP is an Atg8a-interacting protein that regulates Golgi turnover in Drosophila. Cell Rep 39:110903 (2022). PubMed: 35649355
- Delventhal R et al. Dietary restriction ameliorates TBI-induced phenotypes in Drosophila melanogaster. Sci Rep 12:9523 (2022). PubMed: 35681073
- Tao X et al. Phenylbutyrate modulates polyamine acetylase and ameliorates Snyder-Robinson syndrome in a Drosophila model and patient cells. JCI Insight 7:N/A (2022). PubMed: 35801587