Anti-RECQ1 抗体 (ab272855)
Key features and details
- Rabbit polyclonal to RECQ1
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RECQ1 antibody
RECQ1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RECQ1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, IPmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Human RECQ1 (N terminal). The exact sequence is proprietary.
Database link: P46063 -
ポジティブ・コントロール
- WB: A431, HeLa and A375 whole cell lysate. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells. IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272855の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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IP |
1/100 - 1/500.
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特記事項 |
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WB
1/500 - 1/3000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/500. |
ターゲット情報
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機能
DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. -
組織特異性
High expression in heart, lung, skeletal muscle and kidney, low expression in brain. -
配列類似性
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5965 Human
- Omim: 600537 Human
- SwissProt: P46063 Human
- Unigene: 235069 Human
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別名
- ATP dependent DNA helicase Q1 antibody
- ATP-dependent DNA helicase Q1 antibody
- DNA helicase antibody
see all
画像
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All lanes : Anti-RECQ1 antibody (ab272855) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : A-375 (human malignant melanoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.7.5% SDS-PAGE.
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Paraffin-embedded human breast carcinoma tissue stained for RECQ1 using ab272855 at 1/250 dilution in immunohistochemical analysis.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15 mins. -
Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling RECQ1 (green) using ab272855 at 1/500 dilution.
Counterstain: Phalloidin, a cytoskeleton marker (red).
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Immunoprecipitation of RECQ1 protein from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab272855 at 5 μg. ab272855 also used for WB followed by an anti-Rabbit IgG-HRP secondary.
Lane 1: Control IgG.
Lane 2: ab272855 IP in HeLa whole cell lysate.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272855 は論文での使用が確認できていません。