Recombinant Enhanced GFP protein (His tag) (ab134853)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: = 5.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant Enhanced GFP protein (His tag)
GFP タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE.
ab134853 was expressed in E.coli as soluble protein and was purified using a Ni-NTA column. -
エンドトキシン・レベル
= 5.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Aequorea victoria -
配列
MGDIMGEWGNEIFGAIAGFLGVSKGEELFTGVVPILVELDGDVNGHKFSV SGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYV QERTIFFKDDGNYK TRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQ KNGIKVNFKIRHNI EDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDE LYKSRHRRHRQRSRSRAAARRRRRRRRRHHHHHH -
予測される分子量
33 kDa -
領域
2 to 238 -
タグ
His tag C-Terminus -
配列の追加情報
Second generation monomeric GFP (Enhanced GFP). Constructed with a N-terminal tag of HA2 peptide and C-terminal 9 arginine domain/His Tag.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab134853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Liquid -
備考
This version of GFP is the second generation monomeric green fluorescent protein (Enhanced GFP) that has improved brightness and photostability.
This EGFP-9R, have a single excitation peak centered at about 488 nm, with an emission peak wavelength of 509 nm.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
関連情報
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別名
- GFP
- Green fluorescent protein
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関連性
Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.
Subunit structure: Monomer.
Tissue specificity: Photocytes.
Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
Sequence similarities: Belongs to the GFP family.
Biophysicochemical properties: Absorption: Abs(max)=395 nm
Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab134853 は 5 報の論文で使用されています。
- Mann FA et al. Quantum defects as versatile anchors for carbon nanotube functionalization. Nat Protoc 17:727-747 (2022). PubMed: 35110739
- Hunter P et al. Single-molecule and super-resolved imaging deciphers membrane behavior of onco-immunogenic CCR5. iScience 25:105675 (2022). PubMed: 36561885
- Elguindy MM & Mendell JT NORAD-induced Pumilio phase separation is required for genome stability. Nature 595:303-308 (2021). PubMed: 34108682
- Castro L et al. Kir6.2, the Pore-Forming Subunit of ATP-Sensitive K+ Channels, Is Overexpressed in Human Posttraumatic Brain Contusions. J Neurotrauma 36:165-175 (2019). PubMed: 29737232
- Scherr TF et al. A two-magnet strategy for improved mixing and capture from biofluids. Biomicrofluidics 10:024118 (2016). PubMed: 27158286