Recombinant woodchuck CD80 protein (Active) (Biotin) (ab271373)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: Avi tag C-Terminus, Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant woodchuck CD80 protein (Active) (Biotin)
CD80 タンパク質・ペプチド 製品一覧 -
生理活性
Active in a woodchuck CTLA4:CD80 interaction assay.
Buffer is PBS with 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. The coat protein, woodchuck CTLA4 (wCTLA4), was added ON at 4 deg (various concentrations as indicated). Binding reaction initiated with addition of woodchuck B7-1-biotin (wB7-1-biotin) for 2h at RT. Binding was detected using Strep-HRP generated luminescence.
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精製度
>= 90 % SDS-PAGE. -
発現系
HEK 293 cells -
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Woodchuck -
予測される分子量
52 kDa including tags -
分子量情報
Protein runs at higher MW on SDS-PAGE due to glycosylation. -
領域
30 to 237 -
タグ
Avi tag C-Terminus , Fc tag C-Terminus -
配列の追加情報
C-terminal Avi-Tag fused to the Fc-region of Human IgG1.
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標識
Biotin
特性
Our Abpromise guarantee covers the use of ab271373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
Enzymatically biotin-labeled using Avi-tag™ technology
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. Store In the Dark.
pH: 7.40
Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Activation B7-1 antigen
- B lymphocyte activation antigen B7
- B7
see all -
機能
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
組織特異性
Expressed on activated B-cells, macrophages and dendritic cells. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
画像
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Functional analysis of ab271373 in a woodchuck CTLA4:CD80 interaction assay.
Note: B7-1 = CD80.
Buffer is PBS with 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. The coat protein, woodchuck CTLA4 (wCTLA4), was added ON at 4 deg (various concentrations as indicated). Binding reaction initiated with addition of woodchuck B7-1-biotin (wB7-1-biotin) for 2h at RT. Binding was detected using Strep-HRP generated luminescence.
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SDS-PAGE analysis of ab271373.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271373 は論文での使用が確認できていません。