Key features and details
- Expression system: BTI-TN-5B1-4 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, SDS-PAGE, Functional Studies
製品名Recombinant S. cerevisiae KEX2 protease protein (Active)
KEX2 protease タンパク質・ペプチド 製品一覧
ab96554 from High-5 insect cells contains the same specific activity and recognition sequence specificity as yeast derived KEX2.
1 milligram of recombinant KEX2 contains activity equivalent to at least 40 units of yeast derived KEX2.
Cleaves at the carboxyl side of K/R-R.
精製度> 95 % SDS-PAGE.
>= 95% HPLC analyses.
エンドトキシン・レベル< 1.000 Eu/µg
配列LPVPAPPMDS SLLPVKEAED KLSINDPLFE RQWHLVNPSF PGSDINVLDL WYNNITGAGV VAAIVDDGLD YENEDLKDNF CAEGSWDFND NTNLPKPRLS DDYHGTRCAG EIAAKKGNNF CGVGVGYNAK ISGIRILSGD ITTEDEAASL IYGLDVNDIY SCSWGPADDG RHLQGPSDLV KKALVKGVTE GRDSKGAIYV FASGNGGTRG DNCNYDGYTN SIYSITIGAI DHKDLHPPYS EGCSAVMAVT YSSGSGEYIH SSDINGRCSN SHGGTSAAAP LAAGVYTLLL EANPNLTWRD VQYLSILSAV GLEKNADGDW RDSAMGKKYS HRYGFGKIDA HKLIEMSKTW ENVNAQTWFY LPTLYVSQST NSTEETLESV ITISEKSLQD ANFKRIEHVT VTVDIDTEIR GTTTVDLISP AGIISNLGVV RPRDVSSEGF KDWTFMSVAH WGENGVGDWK IKVKTTENGH RIDFHSWRLK LFGESIDSSK TETFVFGNDK EEVEPAATES TVSQYSASST SISISATSTS SISIGVETSA IPQTTTASTD PDSDPNTP
領域110 to 667
Our Abpromise guarantee covers the use of ab96554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.0555% Calcium chloride, 0.082% Sodium acetate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
再構成The lyophilized protein is stable at room temperature for up to 1 month. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.
- KEX 2
- Kexin KEX2
関連性Kex2 in the yeast Saccharomyces cerevisiae is a transmembrane, Ca2+-dependent serine protease of the subtilisin-like pro-protein convertase (SPC) family with specificity for cleavage after paired basic amino acids. At steady state, Kex2 is predominantly localized in late Golgi compartments and initiates the proteolytic maturation of pro-protein precursors that transit the distal secretory pathway. However, Kex2 localization is not static, and its itinerary apparently involves transiting out of the late Golgi and cycling back from post-Golgi endosomal compartments during its lifetime.
細胞内局在Golgi apparatus; trans-Golgi network; trans-Golgi network membrane; single-pass type I membrane protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.