Recombinant rhesus monkey CD4 protein (Fc Chimera Active) (ab201425)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant rhesus monkey CD4 protein (Fc Chimera Active)
CD4 タンパク質・ペプチド 製品一覧 -
生理活性
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human HIV-1 gp120 (CN54) protein (ab174070) at 10 μg/mL (100 μL/well) can bind ab201425 with a linear range of 0.04-0.31 μg/mL.
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rhesus monkey CD4 (Fc Chimera) (ab201425) at 1 μg/mL (100 μL/well) can bind Ibalizumab with a linear range of 0.4-25 ng/mL.
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精製度
> 95 % SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Rhesus monkey -
配列
KKVVLGKKGDTVELTCNASQKKNTQFHWKNSNQIKILGIQGSFLTKGPSK LSDRADSRKSLWDQGCFSMIIKNLKIEDSDTYICEVENKKEEVELLVFGL TANSDTHLLEGQSLTLTLESPPGSSPSVKCRSPGGKNIQGGRTLSVPQLE RQDSGTWTCTVSQDQKTVEFKIDIVVLAFQKASSTVYKKEGEQVEFSFPP AFTLEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMG KKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQFQEN LTCEVWGPTSPKLTLSLKLENKGTTVSKQAKAVWVLNPEAGMWQCLLSDS GQVLLESNIKVVPTW -
予測される分子量
67 kDa including tags -
領域
26 to 390 -
配列の追加情報
Fused with a Human IgG1 Fc tag at the C-terminus.
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特性
Our Abpromise guarantee covers the use of ab201425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Lyophilized -
備考
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.4
Constituents: 0.61% Tris, 0.75% Glycine, 0.06% Sodium chloride, L-Arginine
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is about 3-5%. Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with sterile deionized water to a concentration of 200 µg/ml.
関連情報
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別名
- CD 4
- CD4
- CD4 (L3T4)
see all -
機能
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
配列類似性
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
細胞内局在
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
画像
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The purity of ab201425 was determined by DTT-reduced (R) SDS-PAGE and staining overnight with Coomassie Blue. DTT-reduced Protein migrates as 67-85 kDa in SDS-PAGE due to glycosylation.
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Immobilized Recombinant Human HIV-1 gp120 (CN54) protein (ab174070) at 10 μg/mL (100 μL/well) can bind ab201425 with a linear range of 0.04-0.31 μg/mL.
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Immobilized Recombinant Rhesus monkey CD4 (Fc Chimera) (ab201425) at 1 μg/mL (100 μL/well) can bind Ibalizumab with a linear range of 0.4-25 ng/mL.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab201425 は論文での使用が確認できていません。