Recombinant rat IL-6 protein (Active) (ab281812)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, HPLC, MS, SDS-PAGE
製品の詳細
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製品名
Recombinant rat IL-6 protein (Active)
IL-6 タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by the dose dependent proliferation of rat splenocytes.
ED50 is ≤0.195 ng/ml, corresponding to a specific activity of 5.13 x 106 units/mg.
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精製度
>= 95 % SDS-PAGE.
>=95% Purity by HPLC -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Rat -
配列
PTSQVRRGDF TEDTTHNRPV YTTSQVGGLI TYVLREILEM RKELCNGNSD CMNSDDALSE NNLKLPEIQR NDGCFQTGYN QEICLLKICS GLLEFRFYLE FVKNNLQDNK KDKARVIQSN TETLVHIFKQ EIKDSYKIVL PTPTSNALLM EKLESQKEWL RTKTIQLILK ALEEFLKVTM RSTRQT -
予測される分子量
22 kDa -
実際の分子量
22 kDa -
分子量情報
Mass determination by ESI-TOF. Predicted MW is 21642.77 Da. (+/- 10 Da by ESI-TOF). Observes MW is 21791.47 Da. Additional masses at 22156.88 and 22522.20 are due to residual O-glycans. -
領域
25 to 211 -
配列の追加情報
N-terminal glycine. Full-length mature chain lacking the signal peptide.
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特性
Our Abpromise guarantee covers the use of ab281812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
HPLC
Mass Spectrometry
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
関連情報
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別名
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
機能
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
関連疾患
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
配列類似性
Belongs to the IL-6 superfamily. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
画像
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Functional analysis of ab281812.
Fully biologically active determined by the dose dependent proliferation of rat splenocytes.
ED50 is ≤0.195 ng/ml, corresponding to a specific activity of 5.13 x 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3385914-1
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SDS-PAGE analysis of ab281812.
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Mass determination by ESI-TOF.
Predicted MW is 21642.77 Da. (+/- 10 Da by ESI-TOF). Observes MW is 21791.47 Da. Additional masses at 22156.88 and 22522.20 are due to residual O-glycans.
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HPLC analysis of ab281812.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab281812 は論文での使用が確認できていません。