Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
製品名Recombinant rat IL-1 beta protein
IL-1 beta タンパク質・ペプチド 製品一覧
精製度> 98 % SDS-PAGE.
Endotoxin level is less than 0.1 ng per g (1EU/g).
タンパク質長Full length protein
配列Recombinant Rat IL-1b is a 17.3 kDa protein containing 153 amino acid residues: MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNG QNISQQ VVFSMSFVQG ETSNDKIPVALGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPNWYISTSQ AEH RPVFLGNSNG RDIVDFTMEP VSS
Our Abpromise guarantee covers the use of ab9788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
備考The ED50, determined by the dose-dependent stimulation of murine D10S cells, is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
Concentration information loading...
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
再構成Reconstitute in water to a concentration of 0.1-1.0mg/ml.
- IFN beta inducing factor
機能Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
組織特異性Expressed in activated monocytes/macrophages (at protein level).
配列類似性Belongs to the IL-1 family.
翻訳後修飾Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
細胞内局在Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab9788 は 2 報の論文で使用されています。
- Zhang M et al. Xanthohumol Attenuated Inflammation and ECM Degradation by Mediating HO-1/C/EBPß Pathway in Osteoarthritis Chondrocytes. Front Pharmacol 12:680585 (2021). PubMed: 34017261
- Guo YJ et al. Red nucleus interleukin-1ß evokes tactile allodynia through activation of JAK/STAT3 and JNK signaling pathways. J Neurosci Res 96:1847-1861 (2018). PubMed: 30216497