Recombinant rat FGF9/GAF protein (ab9745)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant rat FGF9/GAF protein
FGF9/GAF タンパク質・ペプチド 製品一覧 -
生理活性
Determined by dose-dependent ability to reduce tetrazolium salt, WST-8, by dehydrogenase activities of BaF3 cells expressing FGF receptors using Cell Counting Kit-8 (CCK-8).
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精製度
> 95 % SDS-PAGE.
Sterile filtered Greater than 95% pure by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g). -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Rat -
予測される分子量
23 kDa
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特性
Our Abpromise guarantee covers the use of ab9745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
備考
mFGF-9 is fully biologically active when compared to standards. The ED50, as determined by the dose-dependent stimulation of thymidine uptake by BaF3 expressing FGF receptors, is less than 0.5 ng/ml, corresponding to a specific activity of >2 X 106 units/mg. Murine FGF-9 exerts full activity on both murine and human cells. For most in vitro applications, FGF-9 exerts its biological activity in the concentration range of 0.1 to 20.0 ng/ml.Previously labelled as FGF9.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute using 50ul of sterile H20.
関連情報
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別名
- FGF 9
- FGF-9
- FGF9
see all -
機能
May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors. -
組織特異性
Glial cells. -
関連疾患
Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3) [MIM:612961]. Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness. -
配列類似性
Belongs to the heparin-binding growth factors family. -
翻訳後修飾
Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.
N-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab9745 は論文での使用が確認できていません。
