Recombinant rat FGF9/GAF protein (ab9745)

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製品の詳細

  • 製品名

    Recombinant rat FGF9/GAF protein
    FGF9/GAF タンパク質・ペプチド 製品一覧

  • 生理活性

    Determined by dose-dependent ability to reduce tetrazolium salt, WST-8, by dehydrogenase activities of BaF3 cells expressing FGF receptors using Cell Counting Kit-8 (CCK-8).

  • 発現系

    Escherichia coli

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Rat

    • 予測される分子量

      23 kDa

特性

Our Abpromise guarantee covers the use of ab9745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 製品の状態

    Lyophilized

  • 備考

    mFGF-9 is fully biologically active when compared to standards. The ED50, as determined by the dose-dependent stimulation of thymidine uptake by BaF3 expressing FGF receptors, is less than 0.5 ng/ml, corresponding to a specific activity of >2 X 106 units/mg. Murine FGF-9 exerts full activity on both murine and human cells. For most in vitro applications, FGF-9 exerts its biological activity in the concentration range of 0.1 to 20.0 ng/ml.

    Previously labelled as FGF9.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    Reconstitute using 50ul of sterile H20.

関連情報

  • 別名

    • FGF 9
    • FGF-9
    • FGF9
    • FGF9_HUMAN
    • Fibroblast growth factor 9
    • GAF
    • GAF (Glia-activafibroblast growth factor 9 (glia-activating factor)
    • Glia Activating Factor
    • Glia-activating factor
    • HBFG 9
    • HBFG9
    • HBGF-9
    • Heparin-binding growth factor 9
    • MGC119914
    • MGC119915
    • SYNS3
    see all

  • 機能

    May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.

  • 組織特異性

    Glial cells.

  • 関連疾患

    Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3) [MIM:612961]. Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness.

  • 配列類似性

    Belongs to the heparin-binding growth factors family.

  • 翻訳後修飾

    Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.
    N-glycosylated.

  • 細胞内局在

    Secreted.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab9745 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9745 は論文での使用が確認できていません。

レビューと Q&A

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Question

I want to order Anti-FGF9 antibody (ab71395) and Anti-FGF2 antibody (ab106245) and I am looking for the equivalent blocking peptides.If they are available could you please send me the reference number? Do you also offer recombinant FGF9 and 2?

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Answer

Vielen Dank für Ihre Anfrage.

Leider haben wir nicht das passende Blockpeptid für den ab106245 Anti-FGF2 antibody in unserem Katalog, dafür aber das aktive, rekommbinate Protein ab129033:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=129033).

Für den ab71395 Anti-FGF9 antibody haben wir so wohl das korrespondierende Blockpeptid ab86940 Click here (or use the following: https://www.abcam.com/index.html?datasheet=86940) als auch das Protein, in der aktiven (ab78476) oder inaktiven (ab9745) Form:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=9745).

Click here (or use the following: https://www.abcam.com/index.html?datasheet=78476).


Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

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Question

What should this antibody be reconstituted in dH2O?

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Answer

Thank you for your enquiry. The source of this protein recommends reconstituting it in water. It is supplied as 10ug and can therefore be reconstituted at the concentration required. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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