Recombinant rat CERK protein (Active) (ab271469)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: = 65% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant rat CERK protein (Active)
CERK タンパク質・ペプチド 製品一覧 -
生理活性
Specific activity ≥20 pmole/min/μg.
CERK activity was measured at 37oC for 25 min in a buffer (20 mM HEPES pH7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 μM ATP) using a μ-Octylglucoside mixed micellar assay with 100 μM Ceramide.
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精製度
= 65 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Rat -
配列
MGAMGAAEPL QSVLWVKQQR CAVSLEPARA LLRWWRSPEP GPSAPDPDAY SVPVSEIIAV EEKEVHQKQS SNGRWHRMEN PFAFTVHCVK RARHHRWKWA RVTFWSADEQ LCQLWLQTLR ELLENLTSRP KHLLVFINPF GGKGQGKHIY EKKVAPLFTL ASITTEIIIT EHANQAKETL YEINTDSYDG IVCVGGDGMF SEVLHGVIGK TQQSAGVDPN HPRAVLVPST LRIGIIPAGS TDCVCYSTVG TNDAETSALH IIIGDSLAID VSSVHHNSTL LRYSVSLLGY GFYGDLIKDS EKKRWMGLVR YDFSGLKAFL SHQYYEGTVS FLPAQHTVGS PRDQKPCRAG CSVCRQSKRQ LEEEQKKALY GLENAEEVEE WQVVCGKFLA INSTNMSCAC PRSPGGLSPF AHLGDGSSDL ILIRKCSRFN FLRFLIRHTN QEDQFGFTFV EVYRVKKFQF TSKHVEDDDN DLKELEKQKF GQICKDNPPC ACPTSRSSWN CDGEVLHSPA IEVRVHCQLV RLFARGIEEE S -
予測される分子量
61 kDa including tags -
領域
1 to 531 -
タグ
DDDDK tag C-Terminus
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特性
Our Abpromise guarantee covers the use of ab271469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)
DDDDK peptide 100 ug/ml.This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Acylsphingosine kinase
- Ceramide kinase
- Cerk
see all -
機能
Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides. -
組織特異性
High level expression in heart, brain, skeletal muscle, kidney and liver; moderate in peripheral blood leukocytes and thymus; very low in spleen, small intestine, placenta and lung. -
配列類似性
Contains 1 DAGKc domain. -
細胞内局在
Cytoplasm. Membrane. - Information by UniProt
画像
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Functional analysis of ab271469.
Specific activity ≥20 pmole/min/μg.
CERK activity was measured at 37oC for 25 min in a buffer (20 mM HEPES pH7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 μM ATP) using a μ-Octylglucoside mixed micellar assay with 100 μM Ceramide.
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SDS-PAGE analysis of ab271469.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271469 は論文での使用が確認できていません。