Recombinant Rabbit Calreticulin protein (ab15729)

Thank you for your enquiry regarding this Calreticulin protein (ab15729). The MW of this recombinant protein is >47kDa, (however it migrates on SDS-PAGE as an approximately 60-kDa protein band). I would suggest loading 2-5 ug per lane. It will require reducing before running on a western blot. Most antibodies recognise reduced and denatured forms of the protein target in western blotting, unless stated otherwise on our datasheets. I hope this helps!

Dear Dr. Hill, Thank you for your patience. Below is the amino acid sequence for ab15729. This rabbit calreticulin protein carries a myc-tag and his-tag at the C-terminus. I hope this information helps with the Mass Spectroscopy experiments. E P V V Y F K E Q F L D G D G W T E R W I E S K H K S D F G K F V L S S G K F Y G D Q E K D K G L Q T S Q D A R F Y A L S A R F E P F S N K G Q P L V V Q F T V K H E Q N I D C G G G Y V K L F P A G L D Q K D M H G D S E Y N I M F G P D I C G P G T K K V H V I F N Y K G K N V L I N K D I R C K D D E F T H L Y T L I V R P D N T Y E V K I D N S Q V E S G S L E D D W D F L P P K K I K D P D A S K P E D W D E R A K I D D P T D S K P E D W D K P E H I P D P D A K K P E D W D E E M D G E W E P P V I Q N P E Y K G E W K P R Q I D N P D Y K G T W I H P E I D N P E Y S P D A N I Y A Y D S F A V L G L D L W Q V K S G T I F D N F L I T N D E A Y A E E F G N E T W G V T K T A E K Q M K D K Q D E E Q R L K E E E E E K K R K E E E E A E E D E E D K D D K E D E D E D E E D K D E E E E E A A A G Q A K D E L P L E Q K L I S E E D L N S A V D H H H H H H

Thank you for your enquiry and patience. Unfortunately, we don't have additional information regarding this product at this time. If we do receive such information, we will update the online datasheet. We do know that the recombinant calreticulin was expressed in E. coli and purified as His-tag protein. Full length cDNA encoding mature rabbit calreticulin was cloned into pBAD expression vector followed by expression in E. coli, cell lysis and affinity chromatography on Ni-column as described in (Guo et al., 2003). The protein has a C-terminal His-tag and has passed all biochemical tests in the lab to indicate that it is properly folded and functional. The best test for the quality of recombinant calreticulin involves limited proteolysis with trypsin as described in (Corbett et al., 2000). The final product is filter sterilized. Please contact us again if you have any additional questions.

Thank you for your email. The buffer only consists of PBS, and there are no preservatives in the buffer. If you have any more questions, please contact us again.

My apologies for the delay in replying to your enquiry, we have just received the following information from the source of ab15729: "The protein is in PBS and we have not added any Ca or Zn to this solution. One may want to add a small amount of EGTA (0.05 uM) to chelate a residual Ca, if any. If there is any residual Ca present it would be in nanomolar concentration, a typical "contaminant" of any solution that does not contain a chelator. When we are interested in studying Ca effects on the protein we always add some EGTA to make sure we have full control of actual Ca concentration in the protein solution." I hope this information helps, please do not hesitate to contact us if you need further help,

Thank you for your enquiry. Here are some details regarding this protein: Recombinant calreticulin was expressed in E. coli and purified as His-tag protein. Full length cDNA encoding mature rabbit calreticulin was cloned into pBAD expression vector followed by expression in E. coli, cell lysis and affinity chromatography on Ni-column as described in (Guo et al., 2003). The protein has a C-terminal His-tag and has passed all biochemical tests in the lab to indicate that it is properly folded and functional. The best test for the quality of recombinant calreticulin involves limited proteolysis with trypsin as described in (Corbett et al., 2000). The final product is filter sterilized.