Recombinant Protein G (HRP) (ab7460)

Reviews (1)Q&A (1)References (5)
Western blot - Recombinant Protein G (HRP) (ab7460)
  • Dot Blot - Recombinant Protein G (HRP) (ab7460)

Key features and details

  • Expression system: Escherichia coli
  • Suitable for: WB, Dot blot, ELISA, IHC-P

製品の詳細

関連製品

    特性

    Our Abpromise guarantee covers the use of ab7460 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Western blot

      Dot blot

      ELISA

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    • 製品の状態

      Lyophilized

    • 備考

      No reaction was observed against anti-Protein A.
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      Preservative: 0.01% Gentamicin sulphate
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1.5% BSA

    • 再構成
      Reconstitute product with 1ml of deionized water.

    関連情報

    • 別名

      • IgG binding protein G
      • IgG-binding protein G
      • Immunoglobulin G binding protein G [Precursor]
      • spg
      • spg immunoglobulin G-binding protein
      • SPG2_STRSG
      see all

    • 関連性

      Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.

    • 細胞内局在

      Cell Wall and Secreted

    画像

    • Western blot - Recombinant Protein G (HRP) (ab7460)
      Western blot - Recombinant Protein G (HRP) (ab7460)Image courtesy of an anonymous Abreview.
      Used for detection of primary antibody in WB after IP with the same antibody. In this case, IP was with goat anti HP1-alpha ab77256 on Protein G beads, and detection was with goat anti HP1-alpha ab77256, and Protein G HRP ab7460. Detection was very specific, and eliminated heavy and light chains of denatured antibodies.

      See Abreview

    • Dot Blot - Recombinant Protein G (HRP) (ab7460)
      Dot Blot - Recombinant Protein G (HRP) (ab7460)
      Dot Blot analysis of ab7460 Protein G protein (HRP).

      Load: 200ng of ab7460 followed by a 3-fold serial dilution

      Antigens:
      Row 1: Human IgG
      Row 2: Rat IgG
      Row 3: Dog IgG

      Primary antibody: none
      Secondary antibody: Protein G Peroxidase Conjugated at 1/1000 for 60 min at room temperature

      Block: Blocking buffer for 60 min at room temperature

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (5)

    ab7460 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab7460 は 5 報の論文で使用されています。

    • Simhadri VL  et al. Prevalence of Pre-existing Antibodies to CRISPR-Associated Nuclease Cas9 in the USA Population. Mol Ther Methods Clin Dev 10:105-112 (2018). PubMed: 30073181
    • Faburay B  et al. A glycosylated recombinant subunit candidate vaccine consisting of Ehrlichia ruminantium major antigenic protein1 induces specific humoral and Th1 type cell responses in sheep. PLoS One 12:e0185495 (2017). ELISA . PubMed: 28957443
    • Gocha T  et al. Identification and characterization of a novel Sso7d scaffold-based binder against Notch1. Sci Rep 7:12021 (2017). PubMed: 28931897
    • Hjerpe R  et al. UBQLN2 Mediates Autophagy-Independent Protein Aggregate Clearance by the Proteasome. Cell 166:935-949 (2016). PubMed: 27477512
    • Zhang F  et al. Macrophage transcriptional responses following in vitro infection with a highly virulent African swine fever virus isolate. J Virol 80:10514-21 (2006). WB . PubMed: 17041222

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    1-2 of 2 Abreviews or Q&A

    Question

    band of interest is at about 27 kDda where light chain of endogenous IgG (from tissue samples) appears
    send reference for using protein A-HRP or protein G-HRP to not detect the heavy and light chain of endogenous IgG in WB

    Read More
    Answer

    Thank you for contacting us.

    Both protein A-HRP (e.g. ab7456) or protein G-HRP (e.g. ab7460) can be used to not detect the heavy and light chain of endogenous IgG in WB.


    The links to the datasheets for protein A (HRP) and protein G (HRP) - which would both work well with a rabbit primary antibody are here:

    https://www.abcam.com/Protein-A-protein-HRP-ab7456.html
    https://www.abcam.com/Protein-G-protein-HRP-ab7460.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Other (IP+WB) Abreview for Protein G protein (HRP)

     Excellent
    Abreviews
    abreview image
    Application
    Other - Do not use

    Review text: Used for detection of primary antibody in WB after IP with the same antibody. In this case, IP was with goat anti HP1-alpha on Protein G beads, and detection was with goat anti HP1-alpha, and Protein G HRP. Detection was very specific, and eliminated heavy and light chains of denatured antibodies.
    Sample: Human Cell lysate - nuclear

    Primary antibody (in addition to 'Protein G protein (HRP)')
    Primary antibody: Abcam primary antibody: Anti-HP1 alpha antibody - ChIP Grade (ab77256)
    Dilution: 1/2500

    Secondary antibody
    Name: Abcam antibody:Protein G protein (HRP)
    Dilution: 1/10000

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    投稿 Feb 24 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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