Recombinant P. falciparum PfHDAC1 protein (Active) (ab271711)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 58% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant P. falciparum PfHDAC1 protein (Active) -
生理活性
Specific Activity: ≥1.85 pmol/min/µg.Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3, and pf-HDAC1. HDAC reaction was performed for 30 mins at 37°C followed by treatment with 50 µl of HDAC developer for 15 mins at RT. Fluorescence intensity is measured at ex360/em460. -
精製度
>= 58 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Plasmodium falciparum -
配列
MSNRKKVAYF HDPDIGSYYY GAGHPMKPQR IRMTHSLIVS YNLYKYMEVY RPHKSDVNEL TLFHDYEYID FLSSISLENY REFTYQLKRF NVGEATDCPV FDGLFQFQQS CAGASIDGAS KLNHHCADIC VNWSGGLHHA KMSEASGFCY INDIVLGILE LLKYHARVMY IDIDVHHGDG VEEAFYVTHR VMTVSFHKFG DYFPGTGDIT DVGVNHGKYY SVNVPLNDGM TDDAFVDLFK VVIDKCVQTY RPGAIIIQCG ADSLTGDRLG RFNLTIKGHA RCVEHVRSYN IPLLVLGGGG YTIRNVSRCW AYETGVVLNK HHEMPDQISL NDYYDYYAPD FQLHLQPSNI PNYNSPEHLS RIKMKIAENL RHIEHAPGVQ FSYVPPDFFN SDIDDESDKN QYELKDDSGG GRAPGTRAKE HSTTHHLRRK NYDDDFFDLS DRDQSIVPY -
予測される分子量
53 kDa including tags -
領域
1 to 449 -
タグ
His tag C-Terminus , DDDDK tag C-Terminus
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製品の詳細
Recombinant P. falciparum PfHDAC1 protein (Active)
関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab271711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 10% Glycerol (glycerin, glycerine)
90 µg/ml FLAG peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271711 は論文での使用が確認できていません。