Recombinant mouse Wnt3a protein (ab81484)
Key features and details
- Expression system: Cell Culture
- Purity: > 75% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: CellDiff, SDS-PAGE, Cellular Activation, HPLC
製品の詳細
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製品名
Recombinant mouse Wnt3a protein
Wnt3a タンパク質・ペプチド 製品一覧 -
生理活性
The ED50 was determined by its ability to induced alkaline phosphatase production by CCL-226 cells.
The expected ED50 for this effect is 0.4-0.6 ng/ml.
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精製度
> 75 % SDS-PAGE.
Greater than 75% by HPLC analyses. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Cell Culture -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Mouse -
配列
SY PIWWSLAVGP QYSSLSTQPI LCASIPGLVP KQLRFCRNYV EIMPSVAEGV KAGIQECQHQ FRGRRWNCTT VSNSLAIFGP VLDKATRESA FVHAIASAGV AFAVTRSCAE GSAAICGCSS RLQGSPGEGW KWGGCSEDIE FGGMVSREFA DARENRPDAR SAMNRHNNEA GRQAIASHMH LKCKCHGLSG SCEVKTCWWS QPDFRTIGDF LKDKYDSASE MVVEKHRESR GWVETLRPRY TYFKVPTERD LVYYEASPNF CEPNPETGSF GTRDRTCNVS SHGIDGCDLL CCGRGHNART ERRREKCHCV FHWCCYVSCQ ECTRVYDVHT CK -
領域
19 to 352 -
配列の追加情報
ab81484 is a monomeric glycoprotein containing 334 amino acid residues. This protein is the mature full length (aa19-352), it does not contain the signal peptide from aa 1-18.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab81484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Cell Differentiation
SDS-PAGE
Cellular Activation
HPLC
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製品の状態
Lyophilized -
備考
Endotoxin level is less than 0.2 ng per µg (2EU/µg).
Formulation: Sterile filtered through a 0.2 micron filter. Lyophilized from 10mM Sodium Phosphate, pH 7.5 + 500mM NaCl + 0.5% CHAPS.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at 4°C (up to 6 months). The lyophilized protein is stable for a few weeks at room temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 13% CHAPS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute to a concentration of 0.1 mg/mL with ddH2O. Store at 4oC for up to 1 week or dilute further in a buffer containing a carrier protein or stabilizer and store working aliquots at -20oC to -80oC for up to 3 months.
関連情報
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別名
- Protein Wnt 3a Precursor
- Protein Wnt-3a
- Wingless type MMTV integration site family member 3A
see all -
機能
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. -
組織特異性
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. -
配列類似性
Belongs to the Wnt family. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
画像
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Human embryonic stem cells (HUES4) were differentiated as monolayer cultures on matrigel coated dishes using a protocol described in Kroon et al, Nat Biotechnol. (2008). Inhibin beta A (ab50051) and Wnt3a (ab81484) were added to cultures to direct differentiation towards definitive endoderm. This image was taken 3 days after induction, where the definitive endoderm marker FOXA2 (ab40874 in green) is abundant. Dapi staining is shown in blue. Magnification: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab81484 は 15 報の論文で使用されています。
- Balbasi E et al. Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages. Methods Mol Biol 2520:295-307 (2022). PubMed: 34611822
- Cheng K et al. Targeted intestinal deletion of Rho guanine nucleotide exchange factor 7, ßPIX, impairs enterocyte proliferation, villus maturation, and mucosal defenses in mice. Am J Physiol Gastrointest Liver Physiol 320:G627-G643 (2021). PubMed: 33566751
- Liu B et al. Loss of endothelial glucocorticoid receptor promotes angiogenesis via upregulation of Wnt/ß-catenin pathway. Angiogenesis 24:631-645 (2021). PubMed: 33650028
- Gong Q et al. Fractalkine aggravates LPS-induced macrophage activation and acute kidney injury via Wnt/ß-catenin signalling pathway. J Cell Mol Med 25:6963-6975 (2021). PubMed: 34101346
- Zhou C et al. LncRNA PVT1 promotes gemcitabine resistance of pancreatic cancer via activating Wnt/ß-catenin and autophagy pathway through modulating the miR-619-5p/Pygo2 and miR-619-5p/ATG14 axes. Mol Cancer 19:118 (2020). PubMed: 32727463