Application
Western blot
The protein ran at a slightly higher molecular weight than expected (ran around ~16kDa as opposed to the expected 13kDa). It also has fragments around ~10kDa. Literature did not indicate a cleavage site.
The protein is not readily visible in sypro at 100ng load. We visualize it in western blot with goat polyclonal antibody to MCP-1
The protein is not readily visible in sypro at 100ng load. We visualize it in western blot with goat polyclonal antibody to MCP-1
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投稿 Sep 03 2014