Recombinant mouse IL-4 protein (Active) (ab259406)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: Cell Culture, SDS-PAGE, HPLC, Functional Studies, MS
製品の詳細
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製品名
Recombinant mouse IL-4 protein (Active)
IL-4 タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by dose dependent increase in HT-2 cell proliferation.
ED50 is ≤1.04 ng/ml, corresponding to a specific activity of 0.96 x 106 units/mg.
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精製度
>= 95 % SDS-PAGE.
=95% by HPLC -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Mouse -
配列
HIHGCDKNHL REIIGILNEV TGEGTPCTEM DVPNVLTATK NTTESELVCR ASKVLRIFYL KHGKTPCLKK NSSVLMELQR LFRAFRCLDS SISCTMNESK STSLKDFLES LKSIMQMDYS -
予測される分子量
14 kDa -
実際の分子量
14 kDa -
分子量情報
Predicted mass is 13615.11 Da (+/- 10 Da by ESI TOF). Observed mass is 13616.32 Da (and + 80 Da due to phosphorylation) -
領域
21 to 140 -
配列の追加情報
Full-length mature chain lacking the signal peptide, N-terminal glycine.
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特性
Our Abpromise guarantee covers the use of ab259406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Cell Culture
SDS-PAGE
HPLC
Functional Studies
Mass Spectrometry
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 6.00
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
関連情報
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別名
- B cell growth factor 1
- B cell IgG differentiation factor
- B Cell Stimulatory Factor 1
see all -
機能
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. -
関連疾患
Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
配列類似性
Belongs to the IL-4/IL-13 family. -
細胞内局在
Secreted. - Information by UniProt
画像
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Fully biologically active determined by dose dependent increase in HT-2 cell proliferation.
ED50 is ≤1.04 ng/ml, corresponding to a specific activity of 0.96 x 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3372567-1
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SDS-PAGE analysis of ab259406.
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Detarmination of mass by ESI TOF.
Predicted mass is 13615.11 Da (+/- 10 Da by ESI TOF). Observed mass is 13616.32 Da (and + 80 Da due to phosphorylation).
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HPLC analysis of ab259406.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab259406 は 1 報の論文で使用されています。
- Shi L et al. Dioscin ameliorates inflammatory bowel disease by up-regulating miR-125a-5p to regulate macrophage polarization. J Clin Lab Anal 36:e24455 (2022). PubMed: 35524480