Recombinant mouse IL-23 protein (Active) (ab259423)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 0.005 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, HPLC, MS, SDS-PAGE, Cell Culture
製品の詳細
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製品名
Recombinant mouse IL-23 protein (Active)
IL-23 タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active as determined by the dose-dependant secretion of IFN-γ by NK-92 cells.
The ED50 is 0.66 ng/mL corresponding to a specific activity of 1.52 x 106 IU/mg.
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精製度
>= 95 % SDS-PAGE.
Purity by HPLC >=95%. -
エンドトキシン・レベル
< 0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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アミノ酸配列 1
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生物種
Mouse -
予測される分子量
36 kDa -
分子量情報
M + 2.62 Da (Calc. mass 35848.48 Da). MWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS -
領域
23 to 335 -
配列の追加情報
N-terminal glycine. Full-length mature chain lacking the signal peptide.
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アミノ酸配列 2
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生物種
Mouse -
予測される分子量
20 kDa -
分子量情報
M + 0.61 Da (Calc. mass 19716.39 Da). -
領域
22 to 196 -
配列の追加情報
N-terminal glycine. Full-length mature chain lacking the signal peptide.
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特性
Our Abpromise guarantee covers the use of ab259423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
HPLC
Mass Spectrometry
SDS-PAGE
Cell Culture
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製品の状態
Lyophilized -
備考
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 6.00
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose
Buffer lyophilized from.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
関連情報
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別名
- IL 23
- IL 23 A
- IL 23 subunit alpha
see all -
機能
Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. -
組織特異性
Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level). -
配列類似性
Belongs to the IL-6 superfamily. -
発生段階
Expressed by newborns dendritic cells. -
細胞内局在
Secreted. Secreted upon association with IL12B. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab259423 は論文での使用が確認できていません。