Recombinant mouse Flt3 ligand/Flt3L protein (Active) (ab270071)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, MS, Functional Studies
製品の詳細
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製品名
Recombinant mouse Flt3 ligand/Flt3L protein (Active)
Flt3 ligand/Flt3L タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by the dose dependent proliferation of AML-5 cells.
ED50 is ≤2.54 ng/ml, corresponding to a specific activity of 0.39 x 106 units/mg.
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精製度
>= 95 % SDS-PAGE.
=95% by HPLC -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Mouse -
配列
GTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQ -
予測される分子量
19 kDa including tags -
実際の分子量
19 kDa including tags -
分子量情報
Predicted mass is 18570.46 Da (+/- 10 Da by ESI TOF). Observed mass is 18571 Da. Additional masses (glycoforms) observed at +365 (Hex(1)HexNac(1)), +203 (HexNac) & +569 (Hex(1)HexNAc(2)). -
領域
27 to 189 -
配列の追加情報
N-terminal glycine tag. Full-length mature chain lacking the signal peptide.
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特性
Our Abpromise guarantee covers the use of ab270071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
HPLC
Mass Spectrometry
Functional Studies
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose
Buffer lyophilized from.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with phosphate buffered saline. Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product.
関連情報
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別名
- FL
- Flt 3 ligand
- Flt 3L
see all -
機能
Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
画像
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Functional Studies - Recombinant mouse Flt3 ligand/Flt3L protein (ab270071)
Fully biologically active determined by the dose dependent proliferation of AML-5 cells.
ED50 is ≤2.54 ng/ml, corresponding to a specific activity of 0.39 x 106 units/mg.
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SDS-PAGE - Recombinant mouse Flt3 ligand/Flt3L protein (ab270071)SDS-PAGE analysis of ab270071.
The protein is heavily glycosylated and therefore runs as several bands.
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Mass Spectrometry - Recombinant mouse Flt3 ligand/Flt3L protein (ab270071)Mass determination by ESI TOF.
Predicted mass is 18570.46 +/- 10 Da by ESI TOF. Observed mass is 18571 Da. Additional masses (glycoforms) observed at +365 (Hex(1)HexNac(1)), +203 (HexNac) & +569 (Hex(1)HexNAc(2)).
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HPLC - Recombinant mouse Flt3 ligand/Flt3L protein (ab270071)HPLC analysis of ab270071.
Additional peaks at 4.50 and 4.75 minutes correspond to multiple glycoforms.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (0)
ab270071 は論文での使用が確認できていません。
