Recombinant mouse Flt3 ligand/Flt3L protein (Active) (ab270071)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, MS, Functional Studies, Cell Culture
製品の詳細
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製品名
Recombinant mouse Flt3 ligand/Flt3L protein (Active)
Flt3 ligand/Flt3L タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by the dose dependent proliferation of AML-5 cells.
ED50 is ≤2.54 ng/ml, corresponding to a specific activity of 0.39 x 106 units/mg.
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精製度
>= 95 % SDS-PAGE.
=95% by HPLC -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Mouse -
配列
GTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQ -
予測される分子量
19 kDa -
実際の分子量
19 kDa -
分子量情報
Predicted mass is 18570.46 Da (+/- 10 Da by ESI TOF). Observed mass is 18571 Da. -
領域
27 to 189 -
配列の追加情報
N-terminal glycine tag. Full-length mature chain lacking the signal peptide.
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特性
Our Abpromise guarantee covers the use of ab270071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
HPLC
Mass Spectrometry
Functional Studies
Cell Culture
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose
Buffer lyophilized from.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
関連情報
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別名
- FL
- Flt 3 ligand
- Flt 3L
see all -
機能
Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
画像
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Fully biologically active determined by the dose dependent proliferation of AML-5 cells.
ED50 is ≤2.54 ng/ml, corresponding to a specific activity of 0.39 x 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3369520-1
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SDS-PAGE analysis of ab270071.
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HPLC analysis of ab270071.
Additional peaks due to glycosylation.
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Mass determination by ESI TOFPredicted MW is 18570.46 +/- 10Da by ESI-TOF. Observed MW is 18571.49. Additional Peaks due to Chains +Gly + O-glycans.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab270071 は論文での使用が確認できていません。