Recombinant mouse EGF protein (ab72994)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant mouse EGF protein
EGF タンパク質・ペプチド 製品一覧 -
生理活性
The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml.
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精製度
> 95 % SDS-PAGE.
Purity is greater than 97% as determined by reducing and non-reducing SDS-PAGE and analytical HPLC. -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Mouse -
配列
NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR
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特性
Our Abpromise guarantee covers the use of ab72994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with sterile water at a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions. Reconstituted material should be aliquoted and frozen at -20oC. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
関連情報
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別名
- Beta urogastrone
- beta-urogastrone
- EGF
see all -
機能
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). -
組織特異性
Expressed in kidney, salivary gland, cerebrum and prostate. -
関連疾患
Hypomagnesemia 4 -
配列類似性
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats. -
翻訳後修飾
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). -
細胞内局在
Membrane. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab72994 は 1 報の論文で使用されています。
- Peng DH et al. Epidermal growth factor alleviates cerebral ischemia-induced brain injury by regulating expression of neutrophil gelatinase-associated lipocalin. Biochem Biophys Res Commun 524:963-969 (2020). PubMed: 32059851