Recombinant mouse CXCL2 protein (Active) (ab282382)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC, MS
製品の詳細
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製品名
Recombinant mouse CXCL2 protein (Active)
CXCL2 タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active measured by its ability to chemoattract neutrophils.
The ED50 for this effect is ≤11.97 ng/ml corresponding to specific activity of 8.35 × 104 units/mg.
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精製度
>= 95 % SDS-PAGE.
=95% purity by HPLC -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Mouse -
配列
AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKV CLDPEAPLVQ KIIQKILNKG KAN -
予測される分子量
8 kDa -
実際の分子量
8 kDa -
分子量情報
Predicted MW is 7906.36 Da (+/- 10 da by ESI-TOF). Observed MW is 7906.27 Da. -
領域
28 to 100 -
配列の追加情報
N-terminal glycine. Full-length mature chain lacking the signal peptide.
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特性
Our Abpromise guarantee covers the use of ab282382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
HPLC
Mass Spectrometry
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製品の状態
Lyophilized -
備考
For research use only and are not intended for diagnostic or therapeutic use, not for use in humans.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.4
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.428% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
関連情報
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別名
- C-X-C motif chemokine 2
- Chemokine (C X C motif) ligand 2
- Chemokine, CXC motif, ligand 2
see all -
機能
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. -
細胞内局在
Secreted. - Information by UniProt
画像
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Fully biologically active measured by its ability to chemoattract neutrophils.
The ED50 for this effect is ≤11.97 ng/ml corresponding to specific activity of 8.35 × 104 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3391946-1
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SDS-PAGE analysis of ab282382.
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Mass determination by ESI-TOF.
Predicted MW is 7906.36 Da. (+/- 10 Da by ESI-TOF). Observed MW is 7906.27 Da.
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HPLC analysis of ab282382.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab282382 は論文での使用が確認できていません。