Recombinant mouse CD80 protein (Active) (Biotin) (ab271374)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: Avi tag C-Terminus, Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
-
製品名
Recombinant mouse CD80 protein (Active) (Biotin)
CD80 タンパク質・ペプチド 製品一覧 -
生理活性
Mouse CD80 protein activity was performed in an inhibitor screening assay using mouse CTLA4 coated on a 96-well plate at 100 ng/well, 4°C, overnight. Various amounts of ab271374 (0-50 ng) was incubated for 2 hours at room temperature, followed by streptavidin-HRP for 1 hour at room temperature. Chemiluminescence produced by HRP substrate was measured using a chemiluminescence reader.
-
精製度
>= 90 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
生物種
Mouse -
配列
VDEQLSKSVK DKVLLPCRYN SPHEDESEDR IYWQKHDKVV LSVIAGKLKV WPEYKNRTLY DNTTYSLIIL GLVLSDRGTY SCVVQKKERG TYEVKHLALV KLSIKADFST PNITESGNPS ADTKRITCFA SGGFPKPRFS WLENGRELPG INTTISQDPE SELYTISSQL DFNTTRNHTI KCLIKYGDAH VSEDFTWEKP PEDPPDSKN -
予測される分子量
52 kDa including tags -
領域
38 to 246 -
タグ
Avi tag C-Terminus , Fc tag C-Terminus -
配列の追加情報
Extracellular domain fused at the C-terminus of the Fc portion of human IgG1.
-
-
標識
Biotin
関連製品
特性
Our Abpromise guarantee covers the use of ab271374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
Functional Studies
-
製品の状態
Liquid -
備考
Enzymatically biotin-labeled using Avi-tag™ technology
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. Store In the Dark.
pH: 7.40
Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
別名
- Activation B7-1 antigen
- B lymphocyte activation antigen B7
- B7
see all -
機能
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
組織特異性
Expressed on activated B-cells, macrophages and dendritic cells. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
画像
-
Functional Studies - Recombinant mouse CD80 protein (Active) (Biotin) (ab271374)
Mouse CD80 protein activity was performed in an inhibitor screening assay using mouse CTLA4 coated on a 96-well plate at 100 ng/well, 4°C, overnight. Various amounts of ab271374 (0-50 ng) was incubated for 2 hours at room temperature, followed by streptavidin-HRP for 1 hour at room temperature. Chemiluminescence produced by HRP substrate was measured using a chemiluminescence reader.
-
SDS-PAGE - Recombinant mouse CD80 protein (Active) (Biotin) (ab271374)SDS-PAGE analysis of 4 µg ab271374.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (0)
ab271374 は論文での使用が確認できていません。
