Recombinant Mouse CD3 protein (Fc Chimera) (ab220543)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: ELISA, SDS-PAGE
製品の詳細
-
製品名
Recombinant Mouse CD3 protein (Fc Chimera)
CD3 タンパク質・ペプチド 製品一覧 -
生理活性
Immobilized ab220543 at 5 μg/mL (100 μL/well) can bind CD3e Monoclonal Antibody, Biotin with a linear range of 5-156 ng/mL (QC tested).
-
精製度
> 95 % SDS-PAGE.
Protein A purified. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
生物種
Mouse -
配列
DAENIEYKVSISGTSVELTCPLDSDENLKWEKNGQELPQKHDKHLVLQDF SEVEDSGYYVCYTPASNKNTYLYLKARVCEYCVEVD -
予測される分子量
36 kDa including tags -
分子量情報
The protein migrates as 40-43 kDa under reducing condition. -
領域
23 to 108 -
タグ
Fc tag C-Terminus -
配列の追加情報
Fused with a human IgG1 Fc tag at the C-terminus. The protein migrates as 5410-5413 kDa under reducing (R) condition (SDS-PAGE).
-
特性
Our Abpromise guarantee covers the use of ab220543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
ELISA
SDS-PAGE
-
製品の状態
Lyophilized -
備考
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.61% Tris, 5% Trehalose, Glycine, Sodium chloride, L-ArginineThis product is an active protein and may elicit a biological response in vivo, handle with caution.
-
再構成Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab220543 は論文での使用が確認できていません。