Recombinant mouse BAFF protein (ab157285)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant mouse BAFF protein
BAFF タンパク質・ペプチド 製品一覧 -
生理活性
Binds to Human (weak) and mouse BCMA, TACI and BAFF-R.
Mediates splenocyte survival. -
精製度
> 95 % SDS-PAGE. -
エンドトキシン・レベル
< 0.100 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Mouse -
配列
AFQGPEETEQDVDLSAPPAPCLPGCRHSQHDDNGMNLRNIIQDCLQLIAD SDTPTIRKGTYTFVPWLLSFKRGNALEEKENKIVVRQTGYFFIYSQVLYT DPIFAMGHVIQRKKVHVFGDELSLVTLFRCIQNMPKTLPNNSCYSAGIAR LEEGDEIQLAIPRENAQISRNGDDTFFGALKLL -
予測される分子量
17 kDa including tags -
領域
127 to 309 -
タグ
DDDDK tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab157285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
備考
Binds to Human (weak) and mouse BCMA, TACI and BAFF-R.
Mediates splenocyte survival. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20ºC.
Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with 100µl sterile water to a concentration of 0.1 mg/ml. Further dilutions should be made with medium containing 5% fetal calf serum or other carrier protein. After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
関連情報
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別名
- ApoL related ligand TALL 1
- B cell Activating Factor
- B lymphocyte stimulator
see all -
機能
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. -
組織特異性
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing.
N-glycosylated. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
画像
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SDS-PAGE analysis: Lane 1: MWt Marker, Lane 2: 1 μg ab157285, stained with Imperial stain
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ab157285 mediates survival of freshly isolated splenocytes.
Method: On day 0 splenocytes were isolated from a freshly collected C57Bl6 spleen. An aliquot of the splenocytes was analyzed on FACS and gated on the SSC-FSC panel. FACS settings were saved. The rest of the cells was put in culture with media alone or with increasing concentrations of ab157285 as indicated. After three days in culture, cells were harvested and analyzed on FACS with the saved setting. Splenocytes Survival Index (ratio % living/% dead cells) was calculated and plotted.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab157285 は 1 報の論文で使用されています。
- Yarchoan M et al. Effects of B cell-activating factor on tumor immunity. JCI Insight 5:N/A (2020). PubMed: 32434989