Recombinant Human ZCH11 protein (Tagged) (Biotin) (ab271777)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 89% SDS-PAGE
- Tags: DDDDK tag C-Terminus, Avi tag C-Terminus
- Suitable for: SDS-PAGE
製品の詳細
-
製品名
Recombinant Human ZCH11 protein (Tagged) (Biotin)
ZCH11 タンパク質・ペプチド 製品一覧 -
精製度
>= 89 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
SQKQQTCTDNTGDSDDSASGIEDVSDDLSKMKNDESNKENSSEMDYLENA TVIDESALTPEQRLGLKQAEERLERDHIFRLEKRSPEYTNCRYLCKLCLI HIENIQGAHKHIKEKRHKKNILEKQEESELRSLPPPSPAHLAALSVAVIE LAKEHGITDDDLRVRQEIVEEMSKVITTFLPECSLRLYGSSLTRFALKSS DVNIDIKFPPKMNHPDLLIKVLGILKKNVLYVDVESDFHAKVPVVVCRDR KSGLLCRVSAGNDMACLTTDLLTALGKIEPVFIPLVLAFRYWAKLCYIDS QTDGGIPSYCFALMVMFFLQQRKPPLLPCLLGSWIEGFDPKRMDDFQLKG IVEEKFVKWECNSSSATEKNSIAEENKAKADQPKDDTKKTETDNQSNAMK EKHGKSPLALETPNRVSLGQLWLELLKFYTLDFALEEYVICVRIQDILTR ENKNWPKRRIAIEDPFSVKRNVARSLNSQLVYEYVVERFRAAYRYFACPQ TKGGNKSTVDFKKREKGKISNKKPVKSNNMATNGCILLGETTEKINAERE QPVQCDEMDCTSQRCIIDNNNLLVNELDFADHGQDSSSLSTSKSSEIEPK LDKKQDDLAPSETCLKKELSQCNCIDLSKSPDPDKSTGTDCRSNLETESS HQSVCTDTSATSCNCKATEDASDLNDDDNLPTQELYYVFDKFILTSGKPP TIVCSICKKDGHSKNDCPEDFRKIDLKPLPPMTNRFREILDLVCKRCFDE LSPPCSEQHNREQILIGLEKFIQKEYDEKARLCLFGSSKNGFGFRDSDLD ICMTLEGHENAEKLNCKEIIENLAKILKRHPGLRNILPITTAKVPIVKFE HRRSGLEGDISLYNTLAQHNTRMLATYAAIDPRVQYLGYTMKVFAKRCDI GD -
分子量情報
127 KDa by SDS-PAGE -
領域
212 to 1313 -
タグ
DDDDK tag C-Terminus , Avi tag C-Terminus
-
-
標識
Biotin
特性
Our Abpromise guarantee covers the use of ab271777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
-
製品の状態
Liquid -
備考
Enzymatically biotin-labeled using Avi-tag™ technology
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. Store In the Dark.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)
関連情報
-
別名
- DKFZp779C1943
- EC 2.7.7.52
- FLJ42878
see all -
機能
Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. -
配列類似性
Belongs to the DNA polymerase type-B-like family.
Contains 3 CCHC-type zinc fingers.
Contains 2 PAP-associated domains. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Cytoplasm. Translocates into the cytoplasm following treatment of the cell with LPS. - Information by UniProt
画像
-
SDS-PAGE - Recombinant Human ZCH11 protein (Tagged) (Biotin) (ab271777)
SDS-PAGE analysis of 2 µg ab271777.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271777 は論文での使用が確認できていません。
