Recombinant human ZCH11 protein (Tagged) (ab271776)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human ZCH11 protein (Tagged)
ZCH11 タンパク質・ペプチド 製品一覧 -
精製度
>= 90 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
SQKQQTCTDN TGDSDDSASG IEDVSDDLSK MKNDESNKEN SSEMDYLENA TVIDESALTP EQRLGLKQAE ERLERDHIFR LEKRSPEYTN CRYLCKLCLI HIENIQGAHK HIKEKRHKKN ILEKQEESEL RSLPPPSPAH LAALSVAVIE LAKEHGITDD DLRVRQEIVE EMSKVITTFL PECSLRLYGS SLTRFALKSS DVNIDIKFPP KMNHPDLLIK VLGILKKNVL YVDVESDFHA KVPVVVCRDR KSGLLCRVSA GNDMACLTTD LLTALGKIEP VFIPLVLAFR YWAKLCYIDS QTDGGIPSYC FALMVMFFLQ QRKPPLLPCL LGSWIEGFDP KRMDDFQLKG IVEEKFVKWE CNSSSATEKN SIAEENKAKA DQPKDDTKKT ETDNQSNAMK EKHGKSPLAL ETPNRVSLGQ LWLELLKFYT LDFALEEYVI CVRIQDILTR ENKNWPKRRI AIEDPFSVKR NVARSLNSQL VYEYVVERFR AAYRYFACPQ TKGGNKSTVD FKKREKGKIS NKKPVKSNNM ATNGCILLGE TTEKINAERE QPVQCDEMDC TSQRCIIDNN NLLVNELDFA DHGQDSSSLS TSKSSEIEPK LDKKQDDLAP SETCLKKELS QCNCIDLSKS PDPDKSTGTD CRSNLETESS HQSVCTDTSA TSCNCKATED ASDLNDDDNL PTQELYYVFD KFILTSGKPP TIVCSICKKD GHSKNDCPED FRKIDLKPLP PMTNRFREIL DLVCKRCFDE LSPPCSEQHN REQILIGLEK FIQKEYDEKA RLCLFGSSKN GFGFRDSDLD ICMTLEGHEN AEKLNCKEII ENLAKILKRH PGLRNILPIT TAKVPIVKFE HRRSGLEGDI SLYNTLAQHN TRMLATYAAI DPRVQYLGYT MKVFAKRCDI GDASRGSLSS YAYILMVLYF LQQRKPPVIP VLQEIFDGKQ IPQRMVDGWN AFFFDKTEEL KKRLPSLGKN TESLGELWLG LLRFYTEEFD FKEYVISIRQ KKLLTTFEKQ WTSKCIAIED PFDLNHNLGA GVSRKMTNFI MKAFINGRKL FGTPFYPLIG REAEYFFDSR VLTDGELAPN DRCCRVCGKI GHYMKDCPKR KS -
予測される分子量
127 kDa including tags -
領域
212 to 1313 -
タグ
DDDDK tag C-Terminus
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特性
Our Abpromise guarantee covers the use of ab271776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)
100 ug/mL DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- DKFZp779C1943
- EC 2.7.7.52
- FLJ42878
see all -
機能
Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. -
配列類似性
Belongs to the DNA polymerase type-B-like family.
Contains 3 CCHC-type zinc fingers.
Contains 2 PAP-associated domains. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Cytoplasm. Translocates into the cytoplasm following treatment of the cell with LPS. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human ZCH11 protein (Tagged) (ab271776)
SDS-PAGE analysis of ab271776.
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Functional Studies - Recombinant Human ZCH11 protein (Tagged) (ab271776)Functional studies of ab271776.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271776 は論文での使用が確認できていません。