Recombinant human Yes1 protein (Active) (ab101504)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human Yes1 protein (Active)
Yes1 タンパク質・ペプチド 製品一覧 -
生理活性
The Specific activity of ab101504 was determined to be 185 nmol/min/mg.
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精製度
> 80 % Densitometry.
The purity was determined to be >80% by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
分子量情報
Approx 91 kDa by SDS-PAGE -
領域
2 to 543 -
タグ
GST tag N-Terminus -
配列の追加情報
GenBank: NM_005433
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関連製品
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab101504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- c Yes
- Cellular Yes 1 protein
- Cellular Yes1 protein
see all -
機能
Promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻訳後修飾
Autophosphorylated. -
細胞内局在
Cytoplasm > cytosol. In epithelial cells infected with Neisseria gonorrhoeae, forms aggregates beneath bacterial microcolonies. - Information by UniProt
画像
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Functional Studies - Recombinant human Yes1 protein (ab101504)The specific activity of Yes1 (ab101504) was determined to be 173.2 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human Yes1 protein (ab101504)SDS PAGE analysis of ab101504 -
SDS-PAGE - Recombinant human Yes1 protein (ab101504)SDS PAGE analysis of ab101504 -
SDS-PAGE - Recombinant human Yes1 protein (ab101504)SDS-PAGE analysis of ab101504. -
Functional Studies - Recombinant human Yes1 protein (ab101504)The Specific activity of ab101504 was determined to be 185 nmol/min/mg.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab101504 は論文での使用が確認できていません。
