Bioactive grade

Recombinant human Wnt1 protein (Active) (ab84080)

製品の詳細

  • 製品名

    Recombinant human Wnt1 protein (Active)
    Wnt1 タンパク質・ペプチド 製品一覧
  • 生理活性

    The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells.

    The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.

  • 精製度

    > 98 % SDS-PAGE.
    >98% HPLC.
  • エンドトキシン・レベル

    < 1.000 Eu/µg
  • 発現系

    Escherichia coli
  • アクセッション番号

  • タンパク質長

    Full length protein
  • Animal free

    No
  • 由来

    Recombinant
    • 生物種

      Human
    • 配列

      ANSSGRWWGI VNVASSTNLL TDSKSLQLVL EPSLQLLSRK QRRLIRQNPG ILHSVSGGLQ SAVRECKWQF RNRRWNCPTA PGPHLFGKIV NRGCRETAFI FAITSAGVTH SVARSCSEGS IESCTCDYRR RGPGGPDWHW GGCSDNIDFG RLFGREFVDS GEKGRDLRFL MNLHNNEAGR TTVFSEMRQE CKCHGMSGSC TVRTCWMRLP TLRAVGDVLR DRFDGASRVL YGNRGSNRAS RAELLRLEPE DPAHKPPSPH DLVYFEKSPN FCTYSGRLGT AGTAGRACNS SSPALDGCEL LCCGRGHRTR TQRVTERCNC TFHWCCHVSC RNCTHTRVLH ECL
    • 予測される分子量

      38 kDa
    • 領域

      28 to 370
    • 配列の追加情報

      Full length mature protein, without the signal peptide.

特性

Our Abpromise guarantee covers the use of ab84080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

    HPLC

  • 製品の状態

    Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    Reconstitute in water to a concentration of 0.1 – 1 mg/ml. ab84080 reconstituted is stable for at least 6 months when stored in working aliquots with a carrier protein such as 0.1% BSA at -20oC. Avoid repeated freeze/thaw cycles.

関連情報

  • 別名

    • BMND16
    • INT1
    • OI15
    • oncogene Int1
    • Proto oncogene protein Wnt 1
    • Proto-oncogene Int-1 homolog
    • Proto-oncogene Wnt-1
    • Wingless type MMTV integration site family member 1
    • wingless-type MMTV integration site family, member 1 (oncogene INT1)
    • Wnt 1
    • wnt1
    • WNT1_HUMAN
    see all
  • 機能

    Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
  • 配列類似性

    Belongs to the Wnt family.
  • 細胞内局在

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

参考文献

This product has been referenced in:

  • Xie W  et al. Sox2 modulates Lef-1 expression during airway submucosal gland development. Am J Physiol Lung Cell Mol Physiol 306:L645-60 (2014). Mouse . Read more (PubMed: 24487391) »
  • Cai WY  et al. The Wnt-ß-catenin pathway represses let-7 microRNA expression through transactivation of Lin28 to augment breast cancer stem cell expansion. J Cell Sci 126:2877-89 (2013). Read more (PubMed: 23613467) »
See all 8 Publications for this product

レビューと Q&A

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

We have validated ab109822 for use with the biotinylated ab84243 and will guarantee these antibodies for use as an sELISA pair. For the positive control, I would recommend using the full length protein, such as ab84080. I hope this helps, please let me know if you need any additional information or assistance.

Read More

Answer

Thank you for your enquiry regarding ab84080. For ab84080, the initial reconstitution should be performed in water to a concentration of 0.1-1.0mg/ml. So, the maximum reconstitution concentration is 1mg/ml (1000ug/ml). I hope this information helps you, and please do not hesitate to contact us if you have further questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

登録