Recombinant human VEGFC protein (ab155739)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human VEGFC protein
VEGFC タンパク質・ペプチド 製品一覧 -
生理活性
Immobilized Human VEGF R3, Fc Tag at 5 μg/mL (100 μL/well) can bind Human VEGF-C, His Tag with a linear range of 2-20 ng/mL.
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精製度
> 95 % SDS-PAGE.
Purified by Immobilized metal affinity chromatography. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
TEETIKFAAAHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGVATNTFF KPPCVSVYRCGGCCNSEGLQCMNTSTSYLSKTLFEITVPLSQGPKPVTIS FANHTSCRCMSKLDVYRQVHSIIRR -
予測される分子量
15 kDa including tags -
領域
103 to 227 -
タグ
His tag C-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab155739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% Trehalose
Normally Mannitol or Trehalose are added as protectants before lyophilisation.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with sterile deionized water to a concentration of 250 µg/ml.
関連情報
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別名
- Flt 4L
- Flt4 ligand
- FLT4 ligand DHM
see all -
機能
Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. -
組織特異性
Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte. -
配列類似性
Belongs to the PDGF/VEGF growth factor family. -
翻訳後修飾
Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing an heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions. -
細胞内局在
Secreted. - Information by UniProt
画像
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Immobilized Human VEGF-C, His Tag at 2 µg/mL (100 µL/well) can bind Biotinylated Human VEGF R1, His,Avitag with a linear range of 0.02-0.625 µg/mL.
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Immobilized Human VEGF R3, Fc Tag at 5 µg/mL (100 µL/well) can bind Human VEGF-C, His Tag with a linear range of 2-20 ng/mL.
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SDS-PAGE analysis of reduced ab155739 stained overnight with Coomassie Blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab155739 は 1 報の論文で使用されています。
- Hou Q et al. Extracellular Hsp90a Promotes Tumor Lymphangiogenesis and Lymph Node Metastasis in Breast Cancer. Int J Mol Sci 22:N/A (2021). PubMed: 34299365