Recombinant human USP9x protein (Tagged) (ab271784)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human USP9x protein (Tagged)
USP9x タンパク質・ペプチド 製品一覧 -
生理活性
Specific Activity: 70 pmol/min/μg
Assay Conditions: 50mM Tris-HCl, 0.5mM EDTA, 0.05% Tween 20, 1mM DTT, 500nM Ubiquitin-AMC. Incubate at room temperature for 30 minutes. Fluorescence intensity is measured at exc/em of 360nm/460nm using Tecan M1000 plate reader.
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精製度
>= 90 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
TATTRGSPVG GNDNQGQAPD GQSQPPLQQN QTSSPDSSNE NSPATPPDEQ GQGDAPPQLE DEEPAFPHTD LAKLDDMINR PRWVVPVLPK GELEVLLEAA IDLSKKGLDV KSEACQRFFR DGLTISFTKI LTDEAVSGWK FEIHRCIINN THRLVELCVA KLSQDWFPLL ELLAMALNPH CKFHIYNGTR PCESVSSSVQ LPEDELFARS PDPRSPKGWL VDLLNKFGTL NGFQILHDRF INGSALNVQI IAALIKPFGQ CYEFLTLHTV KKYFLPIIEM VPQFLENLTD EELKKEAKNE AKNDALSMII KSLKNLASRV PGQEETVKNL EIFRLKMILR LLQISSFNGK MNALNEVNKV ISSVSYYTHR HGNPEEEEWL TAERMAEWIQ QNNILSIVLR DSLHQPQYVE KLEKILRFVI KEKALTLQDL DNIWAAQAGK HEAIVKNVHD LLAKLAWDFS PEQLDHLFDC FKASWTNASK KQREKLLELI RRLAEDDKDG VMAHKVLNLL WNLAHSDDVP VDIMDLALSA HIKILDYSCS QDRDTQKIQW IDRFIEELRT NDKWVIPALK QIREICSLFG EAPQNLSQTQ RSPHVFYRHD LINQLQHNHA LVTLVAENLA TYMESMRLYA RDHEDYDPQT VRLGSRYSHV QEVQERLNFL RFLLKDGQLW LCAPQAKQIW KCLAENAVYL CDREACFKWY SKLMGDEPDL DPDINKDFFE SNVLQLDPSL LTENGMKCFE RFFKAVNCRE GKLVAKRRAY MMDDLELIGL DYLWRVVIQS NDDIASRAID LLKEIYTNLG PRLQVNQVVI HEDFIQSCFD RLKASYDTLC VLDGDKDSVN CARQEAVRMV RVLTVLREYI NECDSDYHEE RTILPMSRAF RGKHLSFVVR FPNQGRQVDD LEVWSHTNDT IGSVRRCILN RIKANVAHTK IELFVGGELI DPADDRKLIG QLNLKDKSLI TAKLTQISSN MPSSPDSSSD SSTGSPGNHG NHYSDGPNPE VESCLPGVIM SLHPRYISFL WQVADLGSSL NMPPLRDGAR VLMKLMPPDS TTIEKLRAIC LDHAKLGESS LSPSLDSLFF GPSASQVLYL TEVVYALLMP AGAPLADDSS DFQFHFLKSG GLPLVLSMLT RNNFLPNADM ETRRGAYLNA LKIAKLLLTA IGYGHVRAVA EACQPGVEGV NPMTQINQVT HDQAVVLQSA LQSIPNPSSE CMLRNVSVRL AQQISDEASR YMPDICVIRA IQKIIWASGC GSLQLVFSPN EEITKIYEKT NAGNEPDLED EQVCCEALEV MTLCFALIPT ALDALSKEKA WQTFIIDLLL HCHSKTVRQV AQEQFFLMCT RCCMGHRPLL FFITLLFTVL GSTARERAKH SGDYFTLLRH LLNYAYNSNI NVPNAEVLLN NEIDWLKRIR DDVKRTGETG IEETILEGHL GVTKELLAFQ TSEKKFHIGC EKGGANLIKE LIDDFIFPAS NVYLQYMRNG ELPAEQAIPV CGSPPTINAG FELLVALAVG CVRNLKQIVD SLTEMYYIGT AITTCEALTE WEYLPPVGPR PPKGFVGLKN AGATCYMNSV IQQLYMIPSI RNGILAIEGT GSDVDDDMSG DEKQDNESNV DPRDDVFGYP QQFEDKPALS KTEDRKEYNI GVLRHLQVIF GHLAASRLQY YVPRGFWKQF RLWGEPVNLR EQHDALEFFN SLVDSLDEAL KALGHPAMLS KVLGGSFADQ KICQGCPHRY ECEESFTTLN VDIRNHQNLL DSLEQYVKGD LLEGANAYHC EKCNKKVDTV KRLLIKKLPP VLAIQLKRFD YDWERECAIK FNDYFEFPRE LDMEPYTVAG VAKLEGDNVN PESQLIQQSE QSESETAGST KYRLVGVLVH SGQASGGHYY SYIIQRNGGD GERNRWYKFD DGDVTECKMD DDEEMKNQCF GGEYMGEVFD HMMKRMSYRR QKRWWNAYIL FYERMDTIDQ DDELIRYISE LAITTRPHQI IMPSAIERSV RKQNVQFMHN RMQYSMEYFQ FMKKLLTCNG VYLNPPPGQD HLLPEAEEIT MISIQLAARF LFTTGFHTKK VVRGSASDWY DALCILLRHS KNVRFWFAHN VLFNVSNRFS EYLLECPSAE VRGAFAKLIV FIAHFSLQDG PCPSPFASPG PSSQAYDNLS LSDHLLRAVL NLLRREVSEH GRHLQQYFNL FVMYANLGVA EKTQLLKLSV PATFMLVSLD EGPGPPIKYQ YAELGKLYSV VSQLIRCCNV SSRMQSSING NPPLPNPFGD PNLSQPIMPI QQNVADILFV RTSYVKKIIE DCSNSEETVK LLRFCCWENP QFSSTVLSEL LWQVAYSYTY ELRPYLDLLL QILLIEDSWQ THRIHNALKG IPDDRDGLFD TIQRSKNHYQ KRAYQCIKCM VALFSNCPVA YQILQGNGDL KRKWTWAVEW LGDELERRPY TGNPQYTYNN WSPPVQSNET SNGYFLERSH SARMTLAKAC ELCPEEEPDD QDAPDEHESP PPEDAPLYPH SPGSQYQQNN HVHGQPYTGP AAHHMNNPQR TGQRAQENYE GSEEVSPPQT KDQ -
予測される分子量
293 kDa including tags -
領域
2 to 2554 -
タグ
His tag N-Terminus , DDDDK tag C-Terminus
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特性
Our Abpromise guarantee covers the use of ab271784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)
100 µg/ml DDDDK peptide,This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Deubiquitinating enzyme FAF X
- Deubiquitinating enzyme FAF-X
- DFFRX
see all -
機能
Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Specifically hydrolyzes both 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. -
組織特異性
Widely expressed in embryonic and adult tissues. -
配列類似性
Belongs to the peptidase C19 family. -
細胞内局在
Cytoplasm. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human USP9x protein (Tagged) (ab271784)
SDS-PAGE analysis of ab271784.
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Functional Studies - Recombinant Human USP9x protein (Tagged) (ab271784)Functional studies of ab271784 was 70 pmol/min/μg.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271784 は論文での使用が確認できていません。
