Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE
製品名Recombinant Human Ube2B protein
Ube2B タンパク質・ペプチド 製品一覧
精製度> 90 % SDS-PAGE.
Purified by using conventional chromatography techniques
タンパク質長Full length protein
配列MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSTP ARRRLMRDFK RLQEDPPVGV SGAPSENNIM QWNAVIFGPE GTPFEDGTFKLVIEFSEEYP NKPPTVRFLS KMFHPNVYAD GSICLDILQN RWSPTYDVSS ILTSIQSLLD EPNPNSPANS QAAQLYQENK REYEKRVSAIVEQSWNDS
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1170)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)
- Anti-Rad6 antibody (ab31917)
- HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
- Anti-6X His tag® antibody [4D11] (ab5000)
Our Abpromise guarantee covers the use of ab95484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
- E2 17kDa
- E2 protein
機能Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
パスウェイProtein modification; protein ubiquitination.
配列類似性Belongs to the ubiquitin-conjugating enzyme family.
細胞内局在Cell membrane. Nucleus. In peripheral neurons, expressed both at the plasma membrane and in nuclei.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.