Recombinant human UBE1 protein (Active) (ab271808)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human UBE1 protein (Active)
UBE1 タンパク質・ペプチド 製品一覧 -
生理活性
Reaction was performed in 50 mM Tris pH 7.4, 5 mM MgCl2, 1 mM DTT, 5 µM ATP, 10 µM Ub, and 0.05% Tween. Reaction was initiated with the addition of UBE1 and was incubated at 30°C for 45 mins. The luminescence was read with the Kinase Glo Max reagent.
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精製度
>= 90 % SDS-PAGE. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
領域
2 to 1058 -
タグ
DDDDK tag N-Terminus
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関連製品
特性
Our Abpromise guarantee covers the use of ab271808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)
100 µg/mL DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- A1S9
- A1S9 protein
- A1S9T
see all -
関連性
Ubiquitinylation, the covalent attachment of ubiquitin to protein, regulates a number of cellular processes including responses to stress. This process is responsible for selective targeting of proteins for degradation. The first step in ubiquitinylation is the activation of ubiquitin by ubiquitin activating enzyme E1 which is a rate-limiting enzyme in the conjugation process. The ubiquitin activating enzyme exists as two isoforms: E1a (117 kDa), localized predominantly in the nucleus, and E1b (110 kDa), localized in the cytoplasm. The first 11 amino acids are considered to be essential for phosphorylation and exclusive nuclear localization of the enzyme. -
細胞内局在
Cytoplasmic and Nuclear
画像
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Functional Studies - Recombinant human UBE1 protein (Active) (ab271808)Specific activity of ab271808.
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SDS-PAGE - Recombinant human UBE1 protein (Active) (ab271808)SDS-PAGE analysis of 2 µg ab271808.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271808 は論文での使用が確認できていません。