Recombinant Human TPPP protein (ab167958)
Key features and details
- Expression system: Escherichia coli
- Purity: > 85% Densitometry
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: WB, ELISA, MS, SDS-PAGE
製品の詳細
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製品名
Recombinant Human TPPP protein -
精製度
> 85 % Densitometry.
ab167958 was purifed using Ni-NTA chromatography and filtered (0.4 µm). -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MKHHHHHHAS MADKAKPAKA ANRTPPKSPG DPSKDRAAKR LSLESEGAGE GAAASPELSA LEEAFRRFAV HGDARATGRE MHGKNWSKLC KDCQVIDGRN VTVTDVDIVF SKIKGKSCRT ITFEQFQEAL EELAKKRFKD KSSEEAVREV HRLIEGKAPI ISGVTKAISS PTVSRLTDTT KFTGSHKERF DPSGKGKGKA GRVDLVDESG YVSGYKHAGT YDQKVQGGK -
予測される分子量
25 kDa including tags -
領域
1 to 219 -
タグ
His tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab167958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
ELISA
Mass Spectrometry
SDS-PAGE
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質量分析
LC-MS/MS -
製品の状態
Lyophilized -
備考
Previously labelled as Tubulin Polymerization Promoting Protein.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -80°C.
Constituents: 99% Phosphate Buffer, 0.43% Sodium chloride
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再構成Add 200 µl of deionized water to prepare a working stock solution of 0.5 mg/ml and let the lyophilized pellet dissolve completely. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage.
Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
関連情報
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別名
- 25 kDa brain specific protein
- 25 kDa brain-specific protein
- Brain specific protein p25 alpha
see all -
機能
May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the TPPP family. -
翻訳後修飾
Poor substrate for GSK3 (By similarity). Phosphorylated by LIMK1 on serine residues. Phosphorylation may alter the tubulin polymerization activity. -
細胞内局在
Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Localizes to glial Lewy bodies in the brains of individuals with synucleinopathies. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab167958 は論文での使用が確認できていません。