Recombinant Human TNFSF12 Protein (Active) (ab283416)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant Human TNFSF12 Protein (Active)
TWEAK タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by its ability to dose dependently proliferate human umbilical vein endothelial cells (HUVECs). ED50 is ≤16.7 ng/ml, corresponding to a specific activity of 6.1x105 units/mg.
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精製度
>= 95 % SDS-PAGE. -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Human -
配列
RSAPKGRKTRARRAIAAHYEVHPRPGQDGAQAGVDGTVSGWEEARINSSS PLRYNRQIGEFIVTRAGLYYLYCQVHFDEGKAVYLKLDLLVDGVLALRCL EEFSATAASSLGPQLRLCQVSGLLALRPGSSLRIRTLPWAHLKAAPFLTY FGLFQVH -
予測される分子量
17 kDa -
領域
93 to 249 -
配列の追加情報
N-terminal glycine Accession: Q4ACW9/O43508
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特性
Our Abpromise guarantee covers the use of ab283416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.4
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with Phosphate Buffered Saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
関連情報
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別名
- APO 3 ligand
- APO 3L
- APO3 ligand
see all -
機能
Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. -
組織特異性
Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain. -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
画像
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Fully biologically active determined by its ability to dose dependently proliferate human umbilical vein endothelial cells (HUVECs). ED50 is ≤16.7 ng/ml, corresponding to a specific activity of 6.1x105 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3398997-2
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SDS-PAGE analysis of ab283416.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab283416 は論文での使用が確認できていません。