Recombinant human Soluble TNF Receptor II protein (ab640)

Submit a review Q&A (1)References (1)

製品の概要

製品の詳細

  • 由来

    Recombinant

  • 由来

    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
      P20333

    • 生物種

      Human

    • 配列

      MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP

    • 分子量

      19 kDa

特性

Our Abpromise guarantee covers the use of ab640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    Determined by its inhibitory effect of the TNF-α mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25 ng/ml of Recombinant Human TNF-α, is 0.125 μg/mL.

  • アプリケーション

    SDS-PAGE

    Functional Studies

  • エンドトキシン・レベル

    < 0.100 Eu/µg

  • 精製度

    > 98 % SDS-PAGE.
    Sterile filtered. Greater than 98% pure by SDS-PAGE and HPLC analyses.

  • 製品の状態

    Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    Reconstitute by adding 200ul dH2O

関連情報

  • 別名

    • CD120b
    • p75
    • p75 TNF receptor
    • p75TNFR
    • p80 TNF alpha receptor
    • p80 TNF-alpha receptor
    • Soluble TNFR1B variant 1
    • TBP-2
    • TBPII
    • TNF R II
    • TNF R2
    • TNF R75
    • TNF-R2
    • TNF-RII
    • TNFBR
    • TNFR-II
    • TNFR1B
    • TNFR2
    • TNFR80
    • Tnfrsf1b
    • TNR1B_HUMAN
    • Tumor necrosis factor beta receptor
    • Tumor necrosis factor binding protein 2
    • Tumor necrosis factor receptor 2
    • Tumor necrosis factor receptor superfamily, member 1B
    • Tumor necrosis factor receptor type II
    • Tumor necrosis factor-binding protein 2
    see all

  • 機能

    Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

  • 配列類似性

    Contains 4 TNFR-Cys repeats.

  • 翻訳後修飾

    Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
    A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.

  • 細胞内局在

    Secreted and Cell membrane.
  • Information by UniProt

参考文献

This product has been referenced in:

  • Dong Y  et al. Essential protective role of tumor necrosis factor receptor 2 in neurodegeneration. Proc Natl Acad Sci U S A 113:12304-12309 (2016). Read more (PubMed: 27791020) »
See 1 Publication for this product

ab640 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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レビューを送る 質問を送る

Question

Kindly clarify why is there difference in molecular weight (80 kDa and 19 kDa)
for human TNF receptor II full length proteins with catalogue numbers ab132049
and ab640, respectively.

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Answer


Ab640 is a fragment of the TNF Receptor II protein. It is comprised of amino acids 33-205 but is an active protein. The activity was determined by its inhibitory effect of the TNF-α mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25 ng/ml of Recombinant Human TNF-α, is 0.125 μg/mL. This 172 amino acid protein runs around 19 kDa in a SDS-PAGE gel.


Ab132049, on the other hand, is a recombinant protein comprised of amino acids 23-461 plus a GST tag (25 kDa). The 80 kDa band size is the result of the 438 amino acid protein plus the 25 kDa tag. This protein has not been shown to have activity. It is only validated for ELISA, WB, and SDS-PAGE.


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