Key features and details
- Expression system: HEK 293 cells
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE
製品名Recombinant human Slit2 protein
Determined by its ability to inhibit MC3T3/E1 osteoblasts cell differentiation.
精製度> 98 % SDS-PAGE.
Purity is greater than 98% by SDS-PAGE gel and HPLC analyses.
発現系HEK 293 cells
配列ACPAQCSCSG STVDCHGLAL RSVPRNIPRN TERLDLNGNN ITRITKTDFA GLRHLRVLQL MENKISTIER GAFQDLKELE RLRLNRNHLQ LFPELLFLGT AKLYRLDLSE NQIQAIPRKA FRGAVDIKNL QLDYNQISCI EDGAFRALRD LEVLTLNNNN ITRLSVASFN HMPKLRTFRL HSNNLYCDCH LAWLSDWLRQ RPRVGLYTQC MGPSHLRGHN VAEVQKREFV CSGHQSFMAP SCSVLHCPAA CTCSNNIVDC RGKGLTEIPT NLPETITEIR LEQNTIKVIP PGAFSPYKKL RRIDLSNNQI SELAPDAFQG LRSLNSLVLY GNKITELPKS LFEGLFSLQL LLLNANKINC LRVDAFQDLH NLNLLSLYDN KLQTIAKGTF SPLRAIQTMH LAQNPFICDC HLKWLADYLH TNPIETSGAR CTSPRRLANK RIGQIKSKKF RCSAKEQYFI PGTEDYRSKL SGDCFADLAC PEKCRCEGTT VDCSNQKLNK IPEHIPQYTA ELRLNNNEFT VLEATGIFKK LPQLRKINFS NNKITDIEEG AFEGASGVNE ILLTSNRLEN VQHKMFKGLE SLKTLMLRSN RITCVGNDSF IGLSSVRLLS LYDNQITTVA PGAFDTLHSL STLNLLANPF NCNCYLAWLG EWLRKKRIVT GNPRCQKPYF LKEIPIQDVA IQDFTCDDGN DDNSCSPLSR CPTECTCLDT VVRCSNKGLK VLPKGIPRDV TELYLDGNQF TLVPKELSNY KHLTLIDLSN NRISTLSNQS FSNMTQLLTL ILSYNRLRCI PPRTFDGLKS LRLLSLHGND ISVVPEGAFN DLSALSHLAI GANPLYCDCN MQWLSDWVKS EYKEPGIARC AGPGEMADKL LLTTPSKKFT CQGPVDVNIL AKCNPCLSNP CKNDGTCNSD PVDFYRCTCP YGFKGQDCDV PIHACISNPC KHGGTCHLKE GEEDGFWCIC ADGFEGENCE VNVDDCEDND CENNSTCVDG INNYTCLCPP EYTGELCEEK LDFCAQDLNP CQHDSKCILT PKGFKCDCTP GYVGEHCDID FDDCQDNKCK NGAHCTDAVN GYTCICPEGY SGLFCEFSPP MV
領域27 to 1118
Our Abpromise guarantee covers the use of ab82131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
再構成For lot specific reconstitution information please contact our Scientific Support Team.
- Drad 1
機能Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.
組織特異性Fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined.
配列類似性Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 7 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 20 LRR (leucine-rich) repeats.
Contains 4 LRRCT domains.
Contains 4 LRRNT domains.
ドメインThe leucine-rich repeat domain is sufficient for guiding both axon projection and neuronal migration, in vitro.
細胞内局在Secreted. The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab82131 は 3 報の論文で使用されています。
- Secq V et al. Stromal SLIT2 impacts on pancreatic cancer-associated neural remodeling. Cell Death Dis 6:e1592 (2015). PubMed: 25590802
- Dai CF et al. Expression and roles of Slit/Robo in human ovarian cancer. Histochem Cell Biol 135:475-85 (2011). PubMed: 21465248
- Tseng RC et al. SLIT2 attenuation during lung cancer progression deregulates beta-catenin and E-cadherin and associates with poor prognosis. Cancer Res 70:543-51 (2010). PubMed: 20068157