Recombinant Human SEMA5A protein (ab151658)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, HPLC
製品の詳細
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製品名
Recombinant Human SEMA5A protein
SEMA5A タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
EAQGTTQCQRTEHPVISYKEIGPWLREFRAKNAVDFSQLTFDPGQKELVV GARNYLFRLQLEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLL VGGDRLFTCGTNAFTPVCTNRSLSNLTEIHDQISGMARCPYSPQHNSTAL LTAGGELYAATAMDFPGRDPAIYRSLGILPPLRTAQYNSKWLNEPNFVSS YDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFM KARLNCSRPGEVPFYYNELQSTFFLPELDLIYGIFTTNVNSIAASAVCVF NLSAIAQAFSGPFKYQENSRSAWLPYPNPNPHFQCGTVDQGLYVNLTERN LQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHVAVDVVQGREALVHIIYL ATDYGTIKKVRVPLNQTSSSCLLEEIELFPERRREPIRSLQILHSQSVLF VGLREHVVKIPLKRCQFYRTRSTCIGAQDPYCGWDVVMKKCTSLEESLSM TQWEQSISACPTRNLTVDGHFGVWSPWTPCTHTDGSAVGSCLCRTRSCDS PAPQCGGWQCEGPGMEIANCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSC SNPTPRHGGRVCVGQNREERYCNEHLLCPPHMFWTGWGPWERCTAQCGGG IQARRRICENGPDCAGCNVEYQSCNTNPCPELKKTTPWTPWTPVNISDNG GHYEQRFRYTCKARLADPNLLEVGRQRIEMRYCSSDGTSGCSTLD -
領域
23 to 765 -
タグ
His tag C-Terminus
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関連製品
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Corresponding Antibody
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Related Products
特性
Our Abpromise guarantee covers the use of ab151658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
HPLC
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製品の状態
Lyophilized -
備考
Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
This product was previously labelled as Semaphorin 5A
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C. Please see notes section.
pH: 7.4
Constituents: 99% Phosphate Buffer, 0.05% Tween, 0.003% EDTA, 0.88% Sodium chloride -
再構成Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
関連情報
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別名
- SEMA5A
- SEMAF
- Semaphorin F
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関連性
Members of the semaphorin protein family are involved in axonal guidance during neural development. Semaphorin 5a is thought to have a bifunctional role in axon guidance, exerting both attractive and inhibitory effects on developing axons associated with limbic function. The thrombospondin repeats of Semaphorin 5a physically interact with both heparan sulfate proteoglycans and chondroitin sulfate proteoglycans, inducing attractive and inhibitory guidance cues, respectively. The biological responses to Semaphorin 5a are induced through binding to its high-affinity receptor, plexin B3, triggering intracellular signaling through Met. -
細胞内局在
Membrane; single-pass type I membrane protein
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab151658 は論文での使用が確認できていません。