Bioactive grade

Recombinant human SDF1 protein (Active) (ab9798)

Submit a review Q&A (2)References (2)

製品の詳細

  • 製品名

    Recombinant human SDF1 protein (Active)
    SDF1 タンパク質・ペプチド 製品一覧

  • 生理活性

    Determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80 ng/ml.

  • 精製度

    >= 98 % SDS-PAGE.
    >= HPLC analyses. Sterile filtered.

  • エンドトキシン・レベル

    < 1.000 Eu/µg

  • 発現系

    Escherichia coli

  • アクセッション番号

    P48061

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

    • 予測される分子量

      8 kDa

    • 領域

      22 to 89

    • 配列の追加情報

      Full length mature protein, minus the signal peptide.

関連製品

特性

Our Abpromise guarantee covers the use of ab9798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    HPLC

    Functional Studies

    SDS-PAGE

  • 製品の状態

    Lyophilized
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    Please add 100ul water to reconstitute this product.

関連情報

  • 別名

    • 12-O-tetradecanoylphorbol 13-acetate repressed protein 1
    • AI174028
    • C-X-C motif chemokine 12
    • Chemokine (C-X-C motif) ligand 12
    • Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)
    • Chemokine CXC motif ligand 12
    • cxcl12
    • hIRH
    • hSDF-1
    • Intercrine reduced in hepatomas
    • IRH
    • OTTHUMP00000019491
    • PBSF
    • Pre-B cell growth-stimulating factor
    • SCYB12
    • SDF 1
    • SDF-1
    • SDF-1-alpha(3-67)
    • SDF-1a
    • SDF-1b
    • SDF1_HUMAN
    • SDF1A
    • SDF1B
    • Stromal cell-derived factor 1
    • Stromal cell-derived factor 1 delta
    • Stromal cell-derived factor 1 gamma
    • Stromal cell-derived factor 1a
    • Stromal cell-derived factor-1 alpha
    • Thymic lymphoma cell-stimulating factor
    • Tlsf
    • TLSF-a
    • TLSF-b
    • Tlsfa
    • Tlsfb
    • TPAR1
    see all

  • 機能

    Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.

  • 組織特異性

    Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.

  • 配列類似性

    Belongs to the intercrine alpha (chemokine CxC) family.

  • 発生段階

    Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney.

  • 翻訳後修飾

    Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.

  • 細胞内局在

    Secreted.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (2)

ab9798 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9798 は 2 報の論文で使用されています。

  • Guo Z  et al. Cordycepin suppresses the migration and invasion of human liver cancer cells by downregulating the expression of CXCR4. Int J Mol Med 45:141-150 (2020). PubMed: 31746344
  • Zhang M  et al. CP-25 exerts anti-angiogenic effects on a rat model of adjuvant-induced arthritis by promoting GRK2-induced downregulation of CXCR4-ERK1/2 signaling in endothelial cells. Mol Med Rep 20:4831-4842 (2019). PubMed: 31661133

レビューと Q&A

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レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Question

I have real time results showing that both cell lines have high
CXCl12 at
the RNA level. for the antibody 9797 last one you gave me. the
western-blot is not at all clear but could be repeated.

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Answer

Thank you for your email.


I am sorry to hear that none of the 3 SDF1 antibodies we have are working for you. Do all 3 antibodies give you the same problem of high background and no band around 10 kDa? How were your samples prepared? And did you use a higher % gel (e.g. 12 or 15%) to prevent smaller MW proteins to run out of the gel?

As we had suggested, we will be happy to offer a credit or refund the original order. Please let me know how you would like to proceed.

I look forward to hear back from you.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Phoned with concern about non-specific band in WB and a lack of band at the correct size. Also inquired about positive controls.

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Answer

Thank you for contacting us.

According to Uniprot, Isoform Alphais ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. It also interacts with the N-terminus of CXCR7.

Using this as a basic for choosing the best positive control, we have tissue lysates in stock for each tissue type:

Liver: ab29889;https://www.abcam.com/Liver-Human-Tissue-Lysate-adult-normal-tissue-ab29889.html

Pancreas: ab29816;https://www.abcam.com/Pancreas-Human-Tissue-Lysate-adult-normal-tissue-ab29816.html

Spleen: ab29699;https://www.abcam.com/Spleen-Human-Tissue-Lysate-adult-normal-tissue-ab29699.html

A recombinant protein is available for positive control as ab9798 (https://www.abcam.com/SDF1-alpha-protein-ab9798.htmlhttps://www.abcam.com/SDF1-alpha-protein-ab9798.html). This is the exact match for the immunogen used to create the antibody.

Another product, ab49124 (https://www.abcam.com/SDF1-antibody-ab49124.html#ab49124wb.jpg), has a nice western blot image using Fetal Lung lysate. This may be a good choice for positive control (ab30282;https://www.abcam.com/Lung-Human-Whole-Cell-Lysate-fetal-normal-tissue-ab30282.html).

Our protein block is ab64226 9https://www.abcam.com/Protein-Block-ab64226.html)

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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