Recombinant Human SAE1 protein (ab96772)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus, T7 tag N-Terminus
- Suitable for: MS, SDS-PAGE
製品の詳細
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製品名
Recombinant Human SAE1 protein
SAE1 タンパク質・ペプチド 製品一覧 -
精製度
> 90 % SDS-PAGE.
ab96772 is purified using conventional chromatography techniques. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MHHHHHHMAS MTGGQQMGRD LYDDDDKDRW GSMVEKEEAG GGISEEEAAQ YDRQIRLWGL EAQKRLRASR VLLVGLKGLG AEIAKNLILA GVKGLTMLDH EQVTPEDPGA QFLIRTGSVG RNRAEASLER AQNLNPMVDV KVDTEDIEKK PESFFTQFDA VCLTCCSRDV IVKVDQICHK NSIKFFTGDV FGYHGYTFAN LGEHEFVEEK TKVAKVSQGV EDGPDTKRAK LDSSETTMVK KKVVFCPVKE ALEVDWSSEK AKAALKRTTS DYFLLQVLLK FRTDKGRDPS SDTYEEDSEL LLQIRNDVLD SLGISPDLLP EDFVRYCFSE MAPVCAVVGG ILAQEIVKAL SQRDPPHNNF FFFDGMKGNG IVECLGPK -
予測される分子量
42 kDa including tags -
領域
1 to 346 -
タグ
His tag N-Terminus , T7 tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab96772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Mass Spectrometry
SDS-PAGE
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質量分析
MALDI-TOF-TOF -
製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine)
関連情報
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別名
- Activator of SUMO1
- AOS1
- HSPC140
see all -
機能
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. -
組織特異性
Expression level increases during S phase and drops in G2 phase (at protein level). -
パスウェイ
Protein modification; protein sumoylation. -
配列類似性
Belongs to the ubiquitin-activating E1 family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab96772 は 1 報の論文で使用されています。
- Zhou LJ et al. The SUMO E3 Ligase MdSIZ1 Targets MdbHLH104 to Regulate Plasma Membrane H+-ATPase Activity and Iron Homeostasis. Plant Physiol 179:88-106 (2019). PubMed: 30333149