Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Tags: DDDDK tag N-Terminus
- Suitable for: WB, SDS-PAGE
製品名Recombinant Human RIG-I/DDX58 protein (Tagged)
RIG-I/DDX58 タンパク質・ペプチド 製品一覧
精製度>= 95 % SDS-PAGE.
発現系HEK 293 cells
タンパク質長Full length protein
領域2 to 925
タグDDDDK tag N-Terminus
Our Abpromise guarantee covers the use of ab271486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
100 µg/ml DDDDK peptide
- DEAD (Asp Glu Ala Asp) box polypeptide 58
機能Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.
組織特異性Present in vascular smooth cells (at protein level).
配列類似性Belongs to the helicase family.
Contains 2 CARD domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
ドメインThe repressor domain controls homomultimerization and interaction with MAVS.
The helicase domain is responsible for dsRNA recognition.
The 2 CARD domains are responsible for interaction with and signaling through MAVS.
The second CARD domain is the primary site for 'Lys-63'-linked ubiquitination.
翻訳後修飾Isgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation.
Ubiquitinated. Undergoes 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS binding and to induce anti-viral signal transduction. Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated ubiquitination. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains.
細胞内局在Cytoplasm. Colocalized with TRIM25 at cytoplasmic perinuclear bodies.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.