Recombinant human RbAp48+EED+SUZ12+AEBP2+EZH2 (mutated A738T) protein (Active) (ab271499)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, DDDDK tag N-Terminus, His tag N-Terminus, His tag N-Terminus, His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human RbAp48+EED+SUZ12+AEBP2+EZH2 (mutated A738T) protein (Active)
RbAp48+EED+SUZ12+AEBP2+EZH2 タンパク質・ペプチド 製品一覧 -
生理活性
Specific Activity: 1.33 pmol/min/μg
Assay conditions: 50 μl reaction mix (20 mM phosphate buffer pH 7.4, 0.05% Tween-20, 10 μM S-adenosylmethionine, and various amount of enzyme complex) add to the wells coated with the substrate. Incubate for 1 hr. Add antibody against methylated K27 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
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精製度
>= 90 % SDS-PAGE. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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アミノ酸配列 1
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生物種
Human -
配列
GQTGKKSEKG PVCWRKRVKS EYMRLRQLKR FRRADEVKSM FSSNRQKILE RTEILNQEWK QRRIQPVHIL TSVSSLRGTR ECSVTSDLDF PTQVIPLKTL NAVASVPIMY SWSPLQQNFM VEDETVLHNI PYMGDEVLDQ DGTFIEELIK NYDGKVHGDR ECGFINDEIF VELVNALGQY NDDDDDDDGD DPEEREEKQK DLEDHRDDKE SRPPRKFPSD KIFEAISSMF PDKGTAEELK EKYKELTEQQ LPGALPPECT PNIDGPNAKS VQREQSLHSF HTLFCRRCFK YDCFLHRKCN YSFHATPNTY KRKNTETALD NKPCGPQCYQ HLEGAKEFAA ALTAERIKTP PKRPGGRRRG RLPNNSSRPS TPTINVLESK DTDSDREAGT ETGGENNDKE EEEKKDETSS SSEANSRCQT PIKMKPNIEP PENVEWSGAE ASMFRVLIGT YYDNFCAIAR LIGTKTCRQV YEFRVKESSI IAPAPAEDVD TPPRKKKRKH RLWAAHCRKI QLKKDGSSNH VYNYQPCDHP RQPCDSSCPC VIAQNFCEKF CQCSSECQNR FPGCRCKAQC NTKQCPCYLA VRECDPDLCL TCGAADHWDS KNVSCKNCSI QRGSKKHLLL APSDVAGWGI FIKDPVQKNE FISEYCGEII SQDEADRRGK VYDKYMCSFL FNLNNDFVVD ATRKGNKIRF ANHSVNPNCY AKVMMVNGDH RIGIFAKRAI QTGEELFFDY RYSQADALKY VGIEREMEIP -
予測される分子量
86 kDa including tags -
領域
2 to 751 -
修飾
mutated A738T -
タグ
His tag N-Terminus -
配列の追加情報
GenBank: NM_004456
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アミノ酸配列 2
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生物種
Human -
配列
SEREVSTAPA GTDMPAAKKQ KLSSDENSNP DLSGDENDDA VSIESGTNTE RPDTPTNTPN APGRKSWGKG KWKSKKCKYS FKCVNSLKED HNQPLFGVQF NWHSKEGDPL VFATVGSNRV TLYECHSQGE IRLLQSYVDA DADENFYTCA WTYDSNTSHP LLAVAGSRGI IRIINPITMQ CIKHYVGHGN AINELKFHPR DPNLLLSVSK DHALRLWNIQ TDTLVAIFGG VEGHRDEVLS ADYDLLGEKI MSCGMDHSLK LWRINSKRMM NAIKESYDYN PNKTNRPFIS QKIHFPDFST RDIHRNYVDC VRWLGDLILS KSCENAIVCW KPGKMEDDID KIKPSESNVT ILGRFDYSQC DIWYMRFSMD FWQKMLALGN QVGKLYVWDL EVEDPHKAKC TTLTHHKCGA AIRQTSFSRD SSILIAVCDD ASIWRWDRLR -
予測される分子量
51 kDa including tags -
領域
2 to 441 -
タグ
DDDDK tag N-Terminus -
配列の追加情報
GenBank: NM_003797
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アミノ酸配列 3
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生物種
Human -
配列
APQKHGGGGG GGSGPSAGSG GGGFGGSAAV AAATASGGKS GGGSCGGGGS YSASSSSSAA AAAGAAVLPV KKPKMEHVQA DHELFLQAFE KPTQIYRFLR TRNLIAPIFL HRTLTYMSHR NSRTNIKRKT FKVDDMLSKV EKMKGEQESH SLSAHLQLTF TGFFHKNDKP SPNSENEQNS VTLEVLLVKV CHKKRKDVSC PIRQVPTGKK QVPLNPDLNQ TKPGNFPSLA VSSNEFEPSN SHMVKSYSLL FRVTRPGRRE FNGMINGETN ENIDVNEELP ARRKRNREDG EKTFVAQMTV FDKNRRLQLL DGEYEVAMQE MEECPISKKR ATWETILDGK RLPPFETFSQ GPTLQFTLRW TGETNDKSTA PIAKPLATRN SESLHQENKP GSVKPTQTIA VKESLTTDLQ TRKEKDTPNE NRQKLRIFYQ FLYNNNTRQQ TEARDDLHCP WCTLNCRKLY SLLKHLKLCH SRFIFNYVYH PKGARIDVSI NECYDGSYAG NPQDIHRQPG FAFSRNGPVK RTPITHILVC RPKRTKASMS EFLESEDGEV EQQRTYSSGH NRLYFHSDTC LPLRPQEMEV DSEDEKDPEW LREKTITQIE EFSDVNEGEK EVMKLWNLHV MKHGFIADNQ MNHACMLFVE NYGQKIIKKN LCRNFMLHLV SMHDFNLISI MSIDKAVTKL REMQQKLEKG ESASPANEEI TEEQNGTANG FSEINSKEKA LETDSVSGVS KQSKKQKL -
予測される分子量
87 kDa including tags -
領域
2 to 739 -
タグ
His tag N-Terminus -
配列の追加情報
GenBank: NM_015355.
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アミノ酸配列 4
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生物種
Human -
配列
AAAITDMADL EELSRLSPLP PGSPGSAARG RAEPPEEEEE EEEEEEEAEA EAVAALLLNG GSGGGGGGGG GGVGGGEAET MSEPSPESAS QAGEDEDEEE DDEEEEDESS SSGGGEEESS AESLVGSSGG SSSDETRSLS PGAASSSSGD GDGKEGLEEP KGPRGSQGGG GGGSSSSSVV SSGGDEGYGT GGGGSSATSG GRRGSLEMSS DGEPLSRMDS EDSISSTIMD VDSTISSGRS TPAMMNGQGS TTSSSKNIAY NCCWDQCQAC FNSSPDLADH IRSIHVDGQR GGVFVCLWKG CKVYNTPSTS QSWLQRHMLT HSGDKPFKCV VGGCNASFAS QGGLARHVPT HFSQQNSSKV SSQPKAKEES PSKAGMNKRR KLKNKRRRSL PRPHDFFDAQ TLDAIRHRAI CFNLSAHIES LGKGHSVVFH STVIAKRKED SGKIKLLLHW MPEDILPDVW VNESERHQLK TKVVHLSKLP KDTALLLDPN IYRTMPQKRL KR -
予測される分子量
53 kDa including tags -
領域
2 to 503 -
タグ
His tag N-Terminus -
配列の追加情報
GenBank: NM_153207
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アミノ酸配列 5
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生物種
Human -
配列
ADKEAAFDDA VEERVINEEY KIWKKNTPFL YDLVMTHALE WPSLTAQWLP DVTRPEGKDF SIHRLVLGTH TSDEQNHLVI ASVQLPNDDA QFDASHYDSE KGEFGGFGSV SGKIEIEIKI NHEGEVNRAR YMPQNPCIIA TKTPSSDVLV FDYTKHPSKP DPSGECNPDL RLRGHQKEGY GLSWNPNLSG HLLSASDDHT ICLWDISAVP KEGKVVDAKT IFTGHTAVVE DVSWHLLHES LFGSVADDQK LMIWDTRSNN TSKPSHSVDA HTAEVNCLSF NPYSEFILAT GSADKTVALW DLRNLKLKLH SFESHKDEIF QVQWSPHNET ILASSGTDRR LNVWDLSKIG EEQSPEDAED GPPELLFIHG GHTAKISDFS WNPNEPWVIC SVSEDNIMQV WQMAENIYND EDPEGSVDPE GQGS -
予測される分子量
48 kDa including tags -
領域
2 to 425 -
タグ
His tag N-Terminus -
配列の追加情報
GenBank: NM_005610
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特性
Our Abpromise guarantee covers the use of ab271499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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細胞内局在
RbAp48: Nucleus. EED: Nucleus. Chromosome. Transiently colocalizes with XIST at inactive X chromosomes. SUZ12: Nucleus. AEBP2: Nucleus. EZH2: Nucleus.
画像
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Functional Studies - Recombinant human RbAp48+EED+SUZ12+AEBP2+EZH2 (mutated A738T) protein (Active) (ab271499)
Specific activity of ab271499 was 1.33 pmol/min/μg.
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SDS-PAGE - Recombinant human RbAp48+EED+SUZ12+AEBP2+EZH2 (mutated A738T) protein (Active) (ab271499)SDS-PAGE analysis of ab271499.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271499 は論文での使用が確認できていません。
