Recombinant human PTP1B protein (Active) (ab51277)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human PTP1B protein (Active)
PTP1B タンパク質・ペプチド 製品一覧 -
生理活性
Specific activity is > 10,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
Activity Assay:- Prepare a 180ul assay buffer into a suitable container: The concentrations are 11mM Tris-HCl pH7.5, 55mM NaCl, 2.2mM DTT, 1.1mM MnCl2, 1.1mM EDTA, 33mM pNPP.
- Equilibrate to 37? and monitor the A405nm until value is constant using a spectrophotometer.
- Add 20ul of recombinant PTP-1B protein 12.5ug/ml in 0.1% BSA/DW.
- Record the absorbance A405nm for 5 minutes.
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精製度
> 95 % SDS-PAGE.
This protein was purified by conventional column chromatographic techniques. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML NRVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYTVRQLEL ENLTTQETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL LEMRKFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE PPPEHIPPPP RPPKRILEPH N -
予測される分子量
37 kDa -
領域
1 to 321 -
配列の追加情報
(NP_002818)
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特性
Our Abpromise guarantee covers the use of ab51277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
Concentration determined by Bradford assay.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.0156% Beta mercaptoethanol, 0.0154% DTT, 0.395% Tris HCl, 0.0292% EDTA, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- PTP1B
- Non receptor tyrosine phosphatase 1
- Protein phosphotyrosylphosphatase 1B
see all -
機能
May play an important role in CKII- and p60c-src-induced signal transduction cascades. -
配列類似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
翻訳後修飾
Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab51277 は 6 報の論文で使用されています。
- Derkach KV et al. [Characterization and biological activity of new 4-oxo-1,4-dihydrocinnoline-based inhibitors of the tyrosine phosphatase PTP1B and TCPTP]. Biomed Khim 68:427-436 (2022). PubMed: 36573409
- Ghosh S et al. ßA1-crystallin regulates glucose metabolism and mitochondrial function in mouse retinal astrocytes by modulating PTP1B activity. Commun Biol 4:248 (2021). PubMed: 33627831
- Wang Y et al. Antioxidant, Anti-Inflammatory, and Antidiabetic Activities of Bioactive Compounds from the Fruits of Livistona chinensis Based on Network Pharmacology Prediction. Oxid Med Cell Longev 2021:7807046 (2021). PubMed: 34707780
- Wu JS et al. New Thiodiketopiperazine and 3,4-Dihydroisocoumarin Derivatives from the Marine-Derived Fungus Aspergillus terreus. Mar Drugs 18:N/A (2020). PubMed: 32110865
- Wu JS et al. Benzyl Furanones and Pyrones from the Marine-Derived Fungus Aspergillus terreus Induced by Chemical Epigenetic Modification. Molecules 25:N/A (2020). PubMed: 32867374
- Zivanovic J et al. Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration. Cell Metab 30:1152-1170.e13 (2019). PubMed: 31735592