Recombinant human PRMT10 protein (Active) (ab271723)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 83% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human PRMT10 protein (Active)
PRMT10 タンパク質・ペプチド 製品一覧 -
生理活性
Assay Description: 10 μl reaction mix containing assay buffer with S-adenosylmethionine, specific PRMT9 substrate, and PRMT9 (30-100 ng) added to the wells. Add antibody against methylated Arg residue and A-screen Acceptor beads. Incubate for 30 mins, then add GSH-conjugated Donor beads followed by Alpha Screening detection.
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精製度
>= 83 % SDS-PAGE. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
SNSRPRSRR DAGGGAGAAG RDELVSRSLQ SAEHCLGVQD FGTAYAHYLL VLSLAPELKH DVKETFQYTL FRWAEELDAL SRIQDLLGCY EQALELFPDD EVICNSMGEH LFRMGFRDEA AGYFHKAVKL NPDFSDAKEN FYRVANWLVE RWHFIMLNDT KRNTIYNAAI QKAVCLGSKS VLDIGAGTGI LSMFAKKAGA HSVYACELSK TMYELACDVV AANKMEAGIK LLHTKSLDIE IPKHIPERVS LVVTETVDAG LFGEGIVESL IHAWEHLLLQ PKTKGESANC EKYGKVIPAS AVIFGMAVEC AEIRRHHRVG IKDIAGIHLP TNVKFQSPAY SSVDTEETIE PYTTEKMSRV PGGYLALTEC FEIMTVDFNN LQELKSLATK KPDKIGIPVI KEGILDAIMV WFVLQLDDEH SLSTSPSEET CWEQAVYPVQ DLADYWIKPG DHVMMEVSCQ DCYLRIQSIS VLGLECEMDV AKSFTQNKDL LSLGNEAELC SALANLQTSK PDAVEQTCIL ESTEIALLNN IPYHEGFKMA MSKVLSSLTP EKLYQTMDTH CQNEMSSGTG QSNTVQNILE PFYVLDVSEG FSVLPVIAGT LGQVKPYSSV EKDQHRIALD LISEANHFPK ETLEFWLRHV EDESAMLQRP KSDKLWSIII LDVIEPSGLI QQEIMEKAAI SRCLLQSGGK IFPQYVLMFG LLVESQTLLE ENAVQGTERT LGLNIAPFIN QFQVPIRVFL DLSSLPCIPL SKPVELLRLD LMTPYLNTSN REVKVYVCKS GRLTAIPFWY HMYLDEEIRL DTSSEASHWK QAAVVLDNPI QVEMGEELVL SIQHHKSNVS ITVKQ -
予測される分子量
97 kDa including tags -
領域
2 to 845 -
タグ
DDDDK tag N-Terminus -
配列の追加情報
C-terminal Strep-Tag
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特性
Our Abpromise guarantee covers the use of ab271723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)
80 µg/ml flag peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- ANM10_HUMAN
- Prmt10
- protein arginine methyltransferase 10 (putative)
- Putative protein arginine N-methyltransferase 10
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配列類似性
Belongs to the protein arginine N-methyltransferase family.
Contains 3 TPR repeats. - Information by UniProt
画像
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Functional Studies - Recombinant human PRMT10 protein (Active) (ab271723)Specific activity of ab271723.
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SDS-PAGE - Recombinant human PRMT10 protein (Active) (ab271723)SDS-PAGE analysis of 2 µg ab271723.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271723 は論文での使用が確認できていません。