Recombinant human PPP2R1A + PP2A-alpha protein (Active) (ab271717)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
-
製品名
Recombinant human PPP2R1A + PP2A-alpha protein (Active) -
生理活性
Specific Activity: ≥100 pmol/min/μg.Assay Conditions: 20 mM HEPES, pH 7.2, 100 mM NaCl, 3 mM DTT, 10 mM pNPP and PP2A. Assay was peformed at 37°C for 40 min, and the reaction progress was measured at 405 nm. Specific activity was calculated based on pNPP= 18,000 M-1cm-1. -
精製度
>= 80 % SDS-PAGE. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
アミノ酸配列 1
-
生物種
Human -
配列
DEKVFTKEL DQWIEQLNEC KQLSESQVKS LCEKAKEILT KESNVQEVRC PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL VALKVRYRER ITILRGNHES RQITQVYGFY DECLRKYGNA NVWKYFTDLF DYLPLTALVD GQIFCLHGGL SPSIDTLDHI RALDRLQEVP HEGPMCDLLW SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA HQLVMEGYNW CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV TRRTPDYFL -
予測される分子量
36 kDa including tags -
領域
2 to 309 -
タグ
His tag C-Terminus
-
アミノ酸配列 2
-
生物種
Human -
配列
AAADGDDSL YPIAVLIDEL RNEDVQLRLN SIKKLSTIAL ALGVERTRSE LLPFLTDTIY DEDEVLLALA EQLGTFTTLV GGPEYVHCLL PPLESLATVE ETVVRDKAVE SLRAISHEHS PSDLEAHFVP LVKRLAGGDW FTSRTSACGL FSVCYPRVSS AVKAELRQYF RNLCSDDTPM VRRAAASKLG EFAKVLELDN VKSEIIPMFS NLASDEQDSV RLLAVEACVN IAQLLPQEDL EALVMPTLRQ AAEDKSWRVR YMVADKFTEL QKAVGPEITK TDLVPAFQNL MKDCEAEVRA AASHKVKEFC ENLSADCREN VIMSQILPCI KELVSDANQH VKSALASVIM GLSPILGKDN TIEHLLPLFL AQLKDECPEV RLNIISNLDC VNEVIGIRQL SQSLLPAIVE LAEDAKWRVR LAIIEYMPLL AGQLGVEFFD EKLNSLCMAW LVDHVYAIRE AATSNLKKLV EKFGKEWAHA TIIPKVLAMS GDPNYLHRMT TLFCINVLSE VCGQDITTKH MLPTVLRMAG DPVANVRFNV AKSLQKIGPI LDNSTLQSEV KPILEKLTQD QDVDVKYFAQ EALTVLSLA -
予測される分子量
92 kDa including tags -
領域
2 to 589 -
タグ
GST tag N-Terminus
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab271717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Functional Studies
SDS-PAGE
-
製品の状態
Liquid -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.49% GlutathioneThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
細胞内局在
PPP2R1A: Cytoplasm. Chromosome > centromere. Centromeric localization requires the presence of BUB1. PP2A-alpha: Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
画像
-
Functional Studies - Recombinant human PPP2R1A + PP2A-alpha protein (Active) (ab271717)
Specific activity of ab271717 was ≥100 pmol/min/μg.
-
SDS-PAGE - Recombinant human PPP2R1A + PP2A-alpha protein (Active) (ab271717)SDS-PAGE analysis of 4 µg ab271717.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab271717 は論文での使用が確認できていません。