Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) (ab220469)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active)
Poliovirus Receptor/PVR タンパク質・ペプチド 製品一覧 -
生理活性
Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220469 with a linear range of 0.1-4 ng/ml.
Measured by its binding ability in a functional ELISA. Immobilized Human DNAM-1, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220469 with a linear range of 1-16 ng/ml.
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精製度
> 95 % SDS-PAGE. -
エンドトキシン・レベル
< 0.100 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
WPPPGTGDVVVQAPTQVPGFLGDSVTLPCYLQVPNMEVTHVSQLTWARHG ESGSMAVFHQTQGPSYSESKRLEFVAARLGAELRNASLRMFGLRVEDEGN YTCLFVTFPQGSRSVDIWLRVLAKPQNTAEVQKVQLTGEPVPMARCVSTG GRPPAQITWHSDLGGMPNTSQVPGFLSGTVTVTSLWILVPSSQVDGKNVT CKVEHESFEKPQLLTVNLTVYYPPEVSISGYDNNWYLGQNEATLTCDARS NPEPTGYNWSTTMGPLPPFAVAQGAQLLIRPVDKPINTTLICNVTNALGA RQAELTVQVKEGPPSEHSGMSRN -
予測される分子量
62 kDa including tags -
領域
21 to 343 -
タグ
Fc tag C-Terminus -
配列の追加情報
Extracellular domain fused with a mouse IgG2a Fc tag at the C-terminus (NP_006496.2).
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特性
Our Abpromise guarantee covers the use of ab220469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
備考
This product was previously labelled as Poliovirus Receptor
After reconstitution this product is stable for 3 months at -80°C under sterile conditions.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.4
Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose, 0.44% L-Arginine, 0.87% Sodium chloride
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
関連情報
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別名
- CD155
- CD155 antigen
- FLJ25946
see all -
機能
Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport. -
配列類似性
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
画像
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FACS analysis shows that the binding of Human CD155, mouse IgG2a Fc Tag to 293T overexpressing TIGIT was inhibited by increasing concentration of neutralizing Anti-Human TIGIT MAb. The concentration of CD155 used is 1 µg/ml. The IC50 is 0.1413 µg/ml.
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FACS assay shows that recombinant Human CD155, mouse IgG2a Fc Tag can bind to 293T cell overexpressing human TIGIT. The concentration of CD155 is 1 µg/ml.
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SDS-PAGE analysis of ab220469 stained overnight with Coomassie Blue.
Lane 1: Reducing conditions.
Lane 2: Non-reducing conditions.
The protein migrates as 70-100 kDa under reducing conditions and 130-160 kDa under non-reducing conditions on SDS-PAGE gel.
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Immobilized Human TIGIT, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220469 with a linear range of 0.1-4 ng/ml.
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Immobilized Human DNAM-1, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220469 with a linear range of 1-16 ng/ml.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab220469 は論文での使用が確認できていません。