Recombinant human Poliovirus Receptor protein (Fc Chimera Active) (ab220469)
製品の概要
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製品名Recombinant human Poliovirus Receptor protein (Fc Chimera Active)
Poliovirus Receptor タンパク質・ペプチド 製品一覧 -
タンパク質長Protein fragment
製品の詳細
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由来Recombinant
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由来HEK 293 cells
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アミノ酸配列
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アクセッション番号
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生物種Human
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配列WPPPGTGDVVVQAPTQVPGFLGDSVTLPCYLQVPNMEVTHVSQLTWARHG ESGSMAVFHQTQGPSYSESKRLEFVAARLGAELRNASLRMFGLRVEDEGN YTCLFVTFPQGSRSVDIWLRVLAKPQNTAEVQKVQLTGEPVPMARCVSTG GRPPAQITWHSDLGGMPNTSQVPGFLSGTVTVTSLWILVPSSQVDGKNVT CKVEHESFEKPQLLTVNLTVYYPPEVSISGYDNNWYLGQNEATLTCDARS NPEPTGYNWSTTMGPLPPFAVAQGAQLLIRPVDKPINTTLICNVTNALGA RQAELTVQVKEGPPSEHSGMSRN
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分子量62 kDa including tags
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領域21 to 343
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配列の追加情報Extracellular domain fused with a mouse IgG2a Fc tag at the C-terminus (NP_006496.2).
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab220469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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生理活性
Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220469 with a linear range of 0.98-7.81 ng/ml.
Measured by its binding ability in a functional ELISA. Immobilized Human DNAM-1, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220469 with a linear range of 0.98-15.63 ng/ml.
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アプリケーション
Functional Studies
SDS-PAGE
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エンドトキシン・レベル< 0.100 Eu/µg
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精製度>95% by SDS-PAGE .
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製品の状態Lyophilised
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
関連情報
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別名
- CD155
- CD155 antigen
- FLJ25946
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機能Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.
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配列類似性Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在Secreted and Cell membrane.
- Information by UniProt
画像
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SDS-PAGE - Recombinant human Poliovirus Receptor protein (Fc Chimera Active) (ab220469)SDS-PAGE analysis of ab220469 stained overnight with Coomassie Blue.
Lane 1: Reducing conditions.
Lane 2: Non-reducing conditions.
The protein migrates as 70-100 kDa under reducing conditions and 130-160 kDa under non-reducing conditions on SDS-PAGE gel.
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Functional Studies - Recombinant human Poliovirus Receptor protein (Fc Chimera Active) (ab220469)Immobilized Human TIGIT, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220469 with a linear range of 0.98-7.81 ng/ml.
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Functional Studies - Recombinant human Poliovirus Receptor protein (Fc Chimera Active) (ab220469)Immobilized Human DNAM-1, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220469 with a linear range of 0.98-15.63 ng/ml.
参考文献
ab220469 has not yet been referenced specifically in any publications.
