Recombinant human PI 3 Kinase p85 alpha protein (Active) (ab271646)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 95% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human PI 3 Kinase p85 alpha protein (Active)
PI 3 Kinase p85 alpha タンパク質・ペプチド 製品一覧 -
生理活性
Specific activity: 1326 pmol/min/μg.
Reactions (25 μl) were performed at 30°C for 15 mins using BPS PI3K substrate, and the samples were incubated with the ADPGlo reagent (25 μl) for 45 mins followed by incubation with the Kinase Detection reagent (50 μl) for 55 mins.
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精製度
>= 95 % SDS-PAGE. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
208 kDa -
領域
1 to 1068 -
タグ
DDDDK tag N-Terminus
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特性
Our Abpromise guarantee covers the use of ab271646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.01% TCEP
80 µg/ml DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- GRB1
- p85
- p85 alpha
see all -
機能
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. -
組織特異性
Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). -
配列類似性
Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
ドメイン
The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef. -
翻訳後修飾
Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
Phosphorylated. Dephosphorylated by PTPRJ. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271646 は論文での使用が確認できていません。