Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 95% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品名Recombinant human PI 3 Kinase p85 alpha protein (Active)
PI 3 Kinase p85 alpha タンパク質・ペプチド 製品一覧
Specific activity: 1326 pmol/min/μg.
Reactions (25 μl) were performed at 30°C for 15 mins using BPS PI3K substrate, and the samples were incubated with the ADPGlo reagent (25 μl) for 45 mins followed by incubation with the Kinase Detection reagent (50 μl) for 55 mins.
精製度>= 95 % SDS-PAGE.
発現系Baculovirus infected Sf9 cells
タンパク質長Full length protein
領域1 to 1068
タグDDDDK tag N-Terminus
Our Abpromise guarantee covers the use of ab271646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.01% TCEP
80 µg/ml DDDDK peptide
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- p85 alpha
機能Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
組織特異性Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
配列類似性Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
ドメインThe SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
翻訳後修飾Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
Phosphorylated. Dephosphorylated by PTPRJ.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.